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Volume 67, Issue 11

sp. nov., a species of the group and potential aquaculture probiont Free

Abstract

Four heterotrophic, antimicrobial, motile, marine bacterial strains, 27-4, 8-1, M6-4.2 and S26, were isolated from aquaculture units in Spain, Denmark and Greece. All four strains produced the antibiotic compound tropodithietic acid, which is a key molecule in their antagonism against fish pathogenic bacteria. Cells of the strains were Gram-reaction-negative, rod-shaped and formed star-shaped aggregates in liquid culture and brown-coloured colonies on marine agar. The predominant cellular fatty acids were C 7, C, C methyl C 7 and C 2-OH, and the polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminolipid, a phospholipid and an unidentified lipid. The strains grew optimally at 31–33 °C. Growth was observed at a salt concentration between 0.5 and 5–6 % NaCl with an optimum at 2–3 %. The pH range for growth of the strains was from pH 6 to 8–8.5 with an optimum at pH 7. Based on 16S rRNA gene sequence analysis, the strains are affiliated with the genus . The genome sequences of the strains have a DNA G+C content of 60.1 % and share an average nucleotide identity (ANI) of more than 95 %. The four strains are distinct from the type strains of the closely related species and based on an ANI of 90.5–91.7 and 89.6–90.4 %, respectively, and an DNA–DNA hybridization relatedness of 43.9–46.9 and 39.8–41.9 %, respectively. On the basis of phylogenetic analyses as well as phenotypic and chemotaxonomic properties, the isolates are considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is 27-4 (=DSM 103509=LMG 29708).

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Keyword(s): aquaculture , Phaeobacter , probiont , Roseobacter group and tropodithietic acid
© 2017 IUMS
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2017-11-01
2024-04-19
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Phaeobacter piscinae sp. nov., a species of the Roseobacter group and potential aquaculture probiont
Int J Syst Evol Microbiol 67, 4559 (2017); https://doi.org/10.1099/ijsem.0.002331
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