%0 Journal Article %A Sonnenschein, Eva Christina %A Phippen, Christopher Broughton William %A Nielsen, Kristian Fog %A Mateiu, Ramona Valentina %A Melchiorsen, Jette %A Gram, Lone %A Overmann, Jörg %A Freese, Heike M. %T Phaeobacter piscinae sp. nov., a species of the Roseobacter group and potential aquaculture probiont %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 11 %P 4559-4564 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002331 %K probiont %K Roseobacter group %K Phaeobacter %K tropodithietic acid %K aquaculture %I Microbiology Society, %X Four heterotrophic, antimicrobial, motile, marine bacterial strains, 27-4T, 8-1, M6-4.2 and S26, were isolated from aquaculture units in Spain, Denmark and Greece. All four strains produced the antibiotic compound tropodithietic acid, which is a key molecule in their antagonism against fish pathogenic bacteria. Cells of the strains were Gram-reaction-negative, rod-shaped and formed star-shaped aggregates in liquid culture and brown-coloured colonies on marine agar. The predominant cellular fatty acids were C18 : 1 ω7c, C16 : 0, C11 methyl C18 : 1 ω7c and C16 : 0 2-OH, and the polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminolipid, a phospholipid and an unidentified lipid. The strains grew optimally at 31–33 °C. Growth was observed at a salt concentration between 0.5 and 5–6 % NaCl with an optimum at 2–3 %. The pH range for growth of the strains was from pH 6 to 8–8.5 with an optimum at pH 7. Based on 16S rRNA gene sequence analysis, the strains are affiliated with the genus Phaeobacter . The genome sequences of the strains have a DNA G+C content of 60.1 % and share an average nucleotide identity (ANI) of more than 95 %. The four strains are distinct from the type strains of the closely related species Phaeobacter gallaeciensis and Phaeobacter inhibens based on an ANI of 90.5–91.7 and 89.6–90.4 %, respectively, and an in silico DNA–DNA hybridization relatedness of 43.9–46.9 and 39.8–41.9 %, respectively. On the basis of phylogenetic analyses as well as phenotypic and chemotaxonomic properties, the isolates are considered to represent a novel species, for which the name Phaeobacter piscinae sp. nov. is proposed. The type strain is 27-4T (=DSM 103509T=LMG 29708T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002331