Phaeobacter piscinae sp. nov., a species of the Roseobacter group and potential aquaculture probiont
Sonnenschein, Eva Christina and Phippen, Christopher Broughton William and Nielsen, Kristian Fog and Mateiu, Ramona Valentina and Melchiorsen, Jette and Gram, Lone and Overmann, Jörg and Freese, Heike M.,, 67, 4559-4564 (2017),
doi = https://doi.org/10.1099/ijsem.0.002331,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Four heterotrophic, antimicrobial, motile, marine bacterial strains, 27-4T, 8-1, M6-4.2 and S26, were isolated from aquaculture units in Spain, Denmark and Greece. All four strains produced the antibiotic compound tropodithietic acid, which is a key molecule in their antagonism against fish pathogenic bacteria. Cells of the strains were Gram-reaction-negative, rod-shaped and formed star-shaped aggregates in liquid culture and brown-coloured colonies on marine agar. The predominant cellular fatty acids were C18 : 1 ω7c, C16 : 0, C11 methyl C18 : 1 ω7c and C16 : 0 2-OH, and the polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminolipid, a phospholipid and an unidentified lipid. The strains grew optimally at 31–33 °C. Growth was observed at a salt concentration between 0.5 and 5–6 % NaCl with an optimum at 2–3 %. The pH range for growth of the strains was from pH 6 to 8–8.5 with an optimum at pH 7. Based on 16S rRNA gene sequence analysis, the strains are affiliated with the genus Phaeobacter . The genome sequences of the strains have a DNA G+C content of 60.1 % and share an average nucleotide identity (ANI) of more than 95 %. The four strains are distinct from the type strains of the closely related species Phaeobacter gallaeciensis and Phaeobacter inhibens based on an ANI of 90.5–91.7 and 89.6–90.4 %, respectively, and an in silico DNA–DNA hybridization relatedness of 43.9–46.9 and 39.8–41.9 %, respectively. On the basis of phylogenetic analyses as well as phenotypic and chemotaxonomic properties, the isolates are considered to represent a novel species, for which the name Phaeobacter piscinae sp. nov. is proposed. The type strain is 27-4T (=DSM 103509T=LMG 29708T).,
language=,
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