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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 12

sp. nov., isolated from wet soil Free

Abstract

A novel aerobic, Gram-stain-negative, rod-shaped, motile bacterium, strain PTW21, was isolated from wet soil. 16S rRNA gene sequence phylogenetic analysis of strain PTW21 revealed an affiliation to the genus and it shared 98.5 and 98.1 % similarity with 5516 S-1 and TS3, respectively. Growth occurred at 10–45 °C, pH 4.5–12.5 and NaCl concentrations up to 2 % (w/v). The major fatty acids were summed feature 3 (Cω and/or iso-C 2-OH) and C. The predominant respiratory quinone was Q-8. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain PTW21 was 64.6 mol%. The results of DNA–DNA hybridization revealed that strain PTW21 showed 37.4 % relatedness with 5516 S-1 and 40.0 % with TS3. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain PTW21 (=CICC 24113=BCRC 81061) is proposed as the type strain of novel species of the genus with the names sp. nov.

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Keyword(s): denitrifying bacterium and Massilia neuiana
© 2017 IUMS
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2017-12-01
2024-04-23
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Massilia neuiana sp. nov., isolated from wet soil
Int J Syst Evol Microbiol 67, 4943 (2017); https://doi.org/10.1099/ijsem.0.002333
/content/journal/ijsem/10.1099/ijsem.0.002333
/content/journal/ijsem/10.1099/ijsem.0.002333
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