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f Puia dinghuensis gen. nov., sp. nov., isolated from monsoon evergreen broad-leaved forest soil

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Puia dinghuensis gen. nov., sp. nov., isolated from monsoon evergreen broad-leaved forest soil, Page 1 of 1

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  • A Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, 4GSH07, was originally isolated from the monsoon evergreen broad-leaved forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31′ E 23° 10′ N). It grew optimally at 28–33 °C and pH 4.0. The 16S rRNA gene sequence analysis showed that strain 4GSH07 had the highest sequence similarity of 94.0 % to Parasegetibacter terrae JCM 19942, and formed an independent lineage separable from other described genera of the family Chitinophagaceae . The main fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 3-OH, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C15 : 0 2-OH. The organism contained MK-7 as the predominant respiratory quinone, and the total DNA G+C content was 50.3 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed consistently that strain 4GSH07 represents a novel species of a novel genus of the family Chitinophagaceae , for which the name Puia dinghuensis gen. nov., sp. nov., is proposed, with 4GSH07 (=CGMCC 1.15448=LMG 29214) as the type strain.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain 4GSH07 is KT805281.

  • Two supplementary tables and four supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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