1887

Abstract

A Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, 4GSH07, was originally isolated from the monsoon evergreen broad-leaved forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31′ E 23° 10′ N). It grew optimally at 28–33 °C and pH 4.0. The 16S rRNA gene sequence analysis showed that strain 4GSH07 had the highest sequence similarity of 94.0 % to JCM 19942, and formed an independent lineage separable from other described genera of the family . The main fatty acids (>5 %) were iso-C, iso-C 3-OH, C 3-OH, summed feature 3 (C 6 and/or C 7) and C 2-OH. The organism contained MK-7 as the predominant respiratory quinone, and the total DNA G+C content was 50.3 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed consistently that strain 4GSH07 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov., is proposed, with 4GSH07 (=CGMCC 1.15448=LMG 29214) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002346
2017-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4639.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002346&mimeType=html&fmt=ahah

References

  1. Kämpfer P, Young CC, Sridhar KR, Arun AB, Lai WA et al. Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov. Int J Syst Evol Microbiol 2006; 56:2223–2228 [View Article][PubMed]
    [Google Scholar]
  2. Kämpfer P, Lodders N, Falsen E. Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov. Int J Syst Evol Microbiol 2011; 61:518–523 [View Article][PubMed]
    [Google Scholar]
  3. Shiratori H, Tagami Y, Morishita T, Kamihara Y, Beppu T et al. Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. Int J Syst Evol Microbiol 2009; 59:1137–1142 [View Article][PubMed]
    [Google Scholar]
  4. Zhang K, Wang Y, Tang Y, Dai J, Zhang L et al. Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella. Int J Syst Evol Microbiol 2010; 60:542–545 [View Article][PubMed]
    [Google Scholar]
  5. Lim JH, Baek SH, Lee ST. Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family 'Chitinophagaceae' in the phylum Bacteroidetes, isolated from freshwater sediment. Int J Syst Evol Microbiol 2009; 59:2394–2399 [View Article][PubMed]
    [Google Scholar]
  6. Albuquerque L, Rainey FA, Nobre MF, da Costa MS. Hydrotalea sandarakina sp. nov., isolated from a hot spring runoff, and emended descriptions of the genus Hydrotalea and the species Hydrotalea flava. Int J Syst Evol Microbiol 2012; 62:1603–1608 [View Article][PubMed]
    [Google Scholar]
  7. Siddiqi MZ, Muhammad Shafi S, Choi KD, Im WT. Compostibacter hankyongensis gen. nov., sp. nov., isolated from compost. Int J Syst Evol Microbiol 2016; 66:3681–3687 [View Article][PubMed]
    [Google Scholar]
  8. Chaudhary DK, Kim J. Arvibacter flaviflagrans gen. nov., sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2016; 66:4347–4354 [View Article][PubMed]
    [Google Scholar]
  9. Lv YY, Wang J, Chen MH, You J, Qiu LH. Dinghuibacter silviterrae gen. nov., sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2016; 66:1785–1791 [View Article][PubMed]
    [Google Scholar]
  10. Zhang L, Chen XL, Hu Q, Chen K, Yan X et al. Haoranjiania flava gen. nov., sp. nov., a new member of the family Chitinophagaceae, isolated from activated sludge. Int J Syst Evol Microbiol 2016; 66:4686–4691 [View Article][PubMed]
    [Google Scholar]
  11. Siddiqi MZ, Muhammad Shafi S, Choi KD, Im WT. Panacibacter ginsenosidivorans gen. nov., sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2016; 66:4039–4045 [View Article][PubMed]
    [Google Scholar]
  12. Kang H, Kim H, Joung Y, Joh K. Parasediminibacterium paludis gen. nov., sp. nov., isolated from wetland. Int J Syst Evol Microbiol 2016; 66:326–331 [View Article][PubMed]
    [Google Scholar]
  13. Siddiqi MZ, Im WT. Pseudobacter ginsenosidimutans gen. nov., sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 2016; 66:3449–3455 [View Article][PubMed]
    [Google Scholar]
  14. Kim SJ, Cho H, Ahn JH, Weon HY, Seok SJ et al. Pseudoflavitalea rhizosphaerae gen. nov., sp. nov., isolated from rhizosphere of tomato, and proposal to reclassify Flavitalea soli as Pseudoflavitalea soli comb. nov. Int J Syst Evol Microbiol 2016; 66:4167–4171 [View Article][PubMed]
    [Google Scholar]
  15. Hiraishi A, Nagashima KV, Matsuura K, Shimada K, Takaichi S et al. Phylogeny and photosynthetic features of Thiobacillus acidophilus and related acidophilic bacteria: its transfer to the genus Acidiphilium as Acidiphilium acidophilum comb. nov. Int J Syst Bacteriol 1998; 48:1389–1398 [View Article][PubMed]
    [Google Scholar]
  16. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics 1991
    [Google Scholar]
  17. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  19. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  20. Doetsch RN. Determinative methods of light microscopy. In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA et al. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 21–33
    [Google Scholar]
  21. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  22. Fautz E, Reichenbach H. A simple test for flexirubin-type pigments. FEMS Microbiol Lett 1980; 8:87–91 [View Article]
    [Google Scholar]
  23. Brown AE. Benson’s Microbiological Applications: Laboratory Manual in General Microbiology, 4th ed. New York: McGraw-Hill; 1985
    [Google Scholar]
  24. Singh PP, Shin YC, Park CS, Chung YR. Biological control of fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 1999; 89:92–99 [View Article][PubMed]
    [Google Scholar]
  25. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum. J Syst Bactericol 1988; 38:358–361 [View Article]
    [Google Scholar]
  26. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
    [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  28. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  29. Weon HY, Kim BY, Yoo SH, Lee SY, Kwon SW et al. Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 2006; 56:1777–1782 [View Article][PubMed]
    [Google Scholar]
  30. Zhang K, Tang Y, Zhang L, Dai J, Wang Y et al. Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. Int J Syst Evol Microbiol 2009; 59:3058–3062 [View Article][PubMed]
    [Google Scholar]
  31. Lim JH, Baek SH, Lee ST. Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family 'Chitinophagaceae' in the phylum Bacteroidetes, isolated from freshwater sediment. Int J Syst Evol Microbiol 2009; 59:2394–2399 [View Article][PubMed]
    [Google Scholar]
  32. Kim BY, Weon HY, Yoo SH, Hong SB, Kwon SW et al. Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. Int J Syst Evol Microbiol 2007; 57:538–541 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002346
Loading
/content/journal/ijsem/10.1099/ijsem.0.002346
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error