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- Volume 67, Issue 12
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f Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.
- Authors: Imen Nouioui1 , Vartul Sangal2 , Lorena Carro1 , Kanae Teramoto3 , Marlen Jando4 , Maria del Carmen Montero-Calasanz1 , José Mariano Igual5 , Iain Sutcliffe2 , Michael Goodfellow1 , Hans-Peter Klenk1
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1 1School of Biology, Ridley Building 2, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK 2 2Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK 3 3Advanced and Fundamental Technology Center, JEOL Ltd., 3–1–2 Musashino, Akishima, Tokyo 196–8558, Japan 4 4Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 5 5Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), c/Cordel de Merinas 40-52, 37008 Salamanca, Spain
- *Correspondence: Imen Nouioui [email protected] [email protected] [email protected]
- First Published Online: 23 October 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4948-4955, doi: 10.1099/ijsem.0.002350
- Subject: New Taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov., Page 1 of 1
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Two rapidly growing mycobacteria with identical 16S rRNA gene sequences were the subject of a polyphasic taxonomic study. The strains formed a well-supported subclade in the mycobacterial 16S rRNA gene tree and were most closely associated with the type strain of Mycobacterium novocastrense . Single and multilocus sequence analyses based on hsp65, rpoB and 16S rRNA gene sequences showed that strains SN 1900T and SN 1904T are phylogenetically distinct but share several chemotaxonomic and phenotypic features that are are consistent with their classification in the genus Mycobacterium . The two strains were distinguished by their different fatty acid and mycolic acid profiles, and by a combination of phenotypic features. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values for strains SN 1900T and SN 1904T were 61.0 % and 94.7 %, respectively; in turn, the corresponding dDDH and ANI values with M. novocastrense DSM 44203T were 41.4 % and 42.8 % and 89.3 % and 89.5 %, respectively. These results show that strains SN1900T and SN 1904T form new centres of taxonomic variation within the genus Mycobacterium . Consequently, strains SN 1900T (40T=CECT 8763T=DSM 43219T) and SN 1904T (2409T=CECT 8766T=DSM 43532T) are considered to represent novel species, for which the names Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov. are proposed. A strain designated as ‘ Mycobacterium acapulsensis’ was shown to be a bona fide member of the putative novel species, M. lehmannii.
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The GenBank accession numbers of strains SN 1900T and SN 1904T for 16S rRNA, hsp65, rpoB gene sequences and genomes are KY933300, KY933786 and KY933788 and KY933299, KY933787 and KY933789, respectively. The genome accession numbers of strains SN 1900T and SN 1904T are NKCN00000000 and NKCO00000000, respectively.
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One supplementary figure is available with the online version of this article.
- Keyword(s): draft-genome sequencing, polyphasic taxonomy, Actinobacteria
© 2017 IUMS | Published by the Microbiology Society
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