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f Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria

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  • Authors: Junwei Zhao1​,† ,  Wenchao Li1​,† ,  Linlin Shi1​ ,  Han Wang1​ ,  Ying Wang1​ ,  Yue Zhao1​ ,  Wensheng Xiang1​,2​ , Xiangjing Wang1​
    • VIEW AFFILIATIONS
    • 1​ 1​Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China 2​ 2​State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
  • *Correspondence: Wensheng Xiang [email protected], Xiangjing Wang [email protected]
  • First Published Online: 27 September 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4655-4659, doi: 10.1099/ijsem.0.002351
  • Subject: New taxa - Actinobacteria
  • Received:
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Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria, Page 1 of 1

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  • A novel actinomycete, designated strain NEAU-A2, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-A2 should be assigned to the genus Lechevalieria and forms a distinct branch with its closest neighbour Lechevalieria aerocolonigenes DSM 40034 (99.0 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain NEAU-A2 to the genus Lechevalieria . The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates were galactose, mannose, rhamnose, glucose and ribose. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannoside and two glycolipids. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 : 0. The DNA G+C content was 68.2 mol%. The combination of the DNA–DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-A2 could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-A2 represents a novel species of the genus Lechevalieria , for which the name Lechevalieria rhizosphaerae sp. nov. is proposed. The type strain is NEAU-A2 (=CGMCC 4.7405=DSM 104541).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NEAU-A2 is KY744941.

  • Four supplementary figures are available with the online Supplementary Material.

© 2017 IUMS | Published by the Microbiology Society

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