@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002376, author = "Okamoto, Rei and Kojima, Hisaya and Fukui, Manabu", title = "Acidocella aquatica sp. nov., a novel acidophilic heterotrophic bacterium isolated from a freshwater lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "11", pages = "4773-4776", doi = "https://doi.org/10.1099/ijsem.0.002376", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002376", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Acidocella", keywords = "freshwater lake", keywords = "acidophile", abstract = "A novel acidophilic heterotrophic bacterium, strain Ok2GT, was isolated from a freshwater lake in Japan. Cells of the isolate were Gram-stain-negative and non-motile rods (0.6–0.8×1.0–2.8 µm). Growth was observed at 4–35 °C with an optimum growth temperature of 28 °C. The pH range for growth was 3.0–6.2 with an optimum pH of 4.5. The strain utilized fructose, glucose, sucrose, mannitol, sorbitol, ethanol, benzyl alcohol, pyruvate, yeast extract and tryptone as carbon and energy sources for aerobic growth. DNA G+C content was 62.6 mol%. The major cellular fatty acid and the isoprenoid quinone were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and Q-10, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain Ok2GT belongs to the genus Acidocella but is distinct from existing species with sequence similarities lower than 97 %. On the basis of these results, strain Ok2GT (=NBRC 112502T=DSM 104037T) is proposed as the type strain of a novel species, Acidocella aquatica sp. nov.", }