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- Volume 67, Issue 11
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f Sphingobacterium tabacisoli sp. nov., isolated from a tobacco field soil sample
- Authors: Xing-Kui Zhou1,† , Qing-Qing Li2,3,† , Ming-He Mo1 , Yong-Guang Zhang4 , Li-Min Dong5 , Min Xiao6 , Wen-Jun Li1,6 , Yan-Qing Duan5
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- VIEW AFFILIATIONS
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1 1State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China 2 2Life Science College, Southwest Forestry University, Kunming 650224, PR China 3 3Kunming Xianghao Technology Co. Ltd., Kunming 650204, PR China 4 4Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China 5 5China Tobacco Yunnan Industrial Co. Ltd., Kunming 650231, PR China 6 6State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China
- *Correspondence: Wen-Jun Li [email protected], Yan-Qing Duan [email protected]
- First Published Online: 06 October 2017, International Journal of Systematic and Evolutionary Microbiology 67: 4808-4813, doi: 10.1099/ijsem.0.002381
- Subject: New taxa - Bacteroidetes
- Received:
- Accepted:
- Cover date:




Sphingobacterium tabacisoli sp. nov., isolated from a tobacco field soil sample, Page 1 of 1
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A Gram-staining-negative, non-motile and rod-shaped bacterium, designated strain h337T, was isolated from an arable soil sample of a tobacco field in Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10–35 °C, at pH 6.0–9.0 and in the presence of up to 3 % (w/v) NaCl, with optimal growth at 30 °C, pH 7.0 and with 1–2 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The cellular polar lipids contained phosphatidylethanolamine, sphingophospholipid, four unidentified phospholipids, five unidentified lipids and three unidentified aminophospholipids. The genomic DNA G+C content was 41.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain h337T should be assigned to the genus Sphingobacterium . 16S rRNA gene sequence similarity analysis showed that strain h337T was most closely related to ‘ Sphingobacterium yamdrokense ’ 3-0-1 (98.8 %) and Sphingobacterium yanglingense CCNWSP36-1T (98.5 %) and shared less than 97 % similarity with other species of the genus Sphingobacterium . DNA–DNA hybridization data indicated that the isolate represented a novel genomic species belonging to the genus Sphingobacterium . The characteristics determined in this polyphasic taxonomic study indicated that strain h337T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium tabacisoli sp. nov. (type strain h337T=KCTC 52298T=CCTCC AB 2017155T) is proposed.
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain h337T is KX129934.
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One supplementary table and three supplementary figures are available with the online Supplementary Material.
© 2017 IUMS | Published by the Microbiology Society
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