1887

Abstract

A Gram-stain-positive, aerobic, endospore-forming, thermophilic bacterium, strain N.8, was isolated from the curing step of an olive mill pomace compost sample, collected at the Composting Experimental Centre (CESCO, Salerno, Italy). Strain N.8, based on 16S rRNA gene sequence similarities, was most closely related to strain H12 (DSM 3670) (99.8 % similarity value) with a 25 % DNA–DNA relatedness value. Cells were rod-shaped, non-motile and grew optimally at 60 °C and pH 9.0, forming cream colonies. Strain N.8 was able to grow on medium containing up to 9.0 % (w/v) NaCl with an optimum at 6.0 % (w/v) NaCl. The cellular membrane contained MK-7, and C (48.4 %), iso-C (19.4 %) and anteiso-C (14.6 %) were the major cellular fatty acids. The DNA GC content was 40.5 mol%. Based on phenotypic characteristics, 16S rRNA gene sequences, DNA–DNA hybridization values and chemotaxonomic characteristics, strain N.8 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is N.8 (=KCTC 33824=JCM 31580).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002391
2017-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4830.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002391&mimeType=html&fmt=ahah

References

  1. Miñana-Galbis D, Pinzón DL, Lorén JG, Manresa A, Oliart-Ros RM. Reclassification of Geobacillus pallidus (Scholz et al. 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov. Int J Syst Evol Microbiol 2010; 60:1600–1604 [View Article][PubMed]
    [Google Scholar]
  2. Scholz T, Demharter W, Hensel R, Kandler O. Bacillus pallidus sp. nov., a new thermophilic species from sewage. Syst Appl Microbiol 1987; 9:91–96 [View Article]
    [Google Scholar]
  3. Banat IM, Marchant R, Rahman TJ. Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. Int J Syst Evol Microbiol 2004; 54:2197–2201 [View Article][PubMed]
    [Google Scholar]
  4. Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans . Int J Syst Evol Microbiol 2001; 51:433–446 [View Article][PubMed]
    [Google Scholar]
  5. Pikuta E, Lysenko A, Chuvilskaya N, Mendrock U, Hippe H et al. Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov. Int J Syst Evol Microbiol 2000; 50:2109–2117 [View Article][PubMed]
    [Google Scholar]
  6. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  7. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009; 59:2114–2121 [View Article][PubMed]
    [Google Scholar]
  13. Dussault HP. An improved technique for staining red halophilic bacteria. J Bacteriol 1955; 70:484–485[PubMed]
    [Google Scholar]
  14. Poli A, Nicolaus B, Denizci AA, Yavuzturk B, Kazan D. Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Camalti Saltern Area, Turkey. Int J Syst Evol Microbiol 2013; 63:10–18 [Crossref]
    [Google Scholar]
  15. Radchenkova N, Vassilev S, Panchev I, Anzelmo G, Tomova I et al. Production and properties of two novel exopolysaccharides synthesized by a thermophilic bacterium Aeribacillus pallidus 418. Appl Biochem Biotechnol 2013; 171:31–43 [View Article][PubMed]
    [Google Scholar]
  16. Rustigian R, Stuart CA. Decomposition of urea by proteus. Exp Biol Med 1941; 47:108–112 [View Article]
    [Google Scholar]
  17. Clark WM, Lubs HA. The differentiation of bacteria of the Colonaerogenes family by the use of indicators. J Infect Dis 1915; 17:160–173 [View Article]
    [Google Scholar]
  18. Poli A, Esposito E, Orlando P, Lama L, Giordano A et al. Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium. Syst Appl Microbiol 2007; 30:31–38 [View Article][PubMed]
    [Google Scholar]
  19. Finore I, Orlando P, di Donato P, Leone L, Nicolaus B et al. Nesterenkonia aurantiaca, sp. nov., an alkaliphilic actinobacterium isolated from Cape King (Antarctica). Int J Syst Evol Microbiol 2016; 66:1554–1560 [Crossref]
    [Google Scholar]
  20. Poli A, Romano I, Cordella P, Orlando P, Nicolaus B et al. Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy. Extremophiles 2009; 13:867–874 [View Article][PubMed]
    [Google Scholar]
  21. Romano I, Dipasquale L, Orlando P, Lama L, D'Ippolito G et al. Thermoanaerobacterium thermostercus sp. nov., a new anaerobic thermophilic hydrogen-producing bacterium from buffalo-dung. Extremophiles 2010; 14:233–240 [View Article][PubMed]
    [Google Scholar]
  22. Romano I, Lama L, Orlando P, Nicolaus B, Giordano A et al. Halomonas sinaiensis sp. nov., a novel halophilic bacterium isolated from a salt lake inside Ras Muhammad Park, Egypt. Extremophiles 2007; 11:789–796 [View Article][PubMed]
    [Google Scholar]
  23. Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266 [View Article][PubMed]
    [Google Scholar]
  24. Nicolaus B, Manca MC, Lama L, Esposito E, Gambacorta A. Lipid modulation by environmental stresses in two models of extremophiles isolated from Antarctica. Polar Biol 2001; 24:1–8 [View Article]
    [Google Scholar]
  25. Poli A, Laezza G, Gul-Guven R, Orlando P, Nicolaus B. Geobacillus galactosidasius sp. nov., a new thermophilic galactosidase-producing bacterium isolated from compost. Syst Appl Microbiol 2011; 34:419–423 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002391
Loading
/content/journal/ijsem/10.1099/ijsem.0.002391
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Supplementary File 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error