Lentzea pudingi sp. nov., isolated from a weathered limestone sample in a karst area Cao, Chengliang and Yuan, Bo and Qin, Sheng and Jiang, Jihong and Tao, Faxiang and Lian, Bin,, 67, 4873-4878 (2017), doi = https://doi.org/10.1099/ijsem.0.002400, publicationName = Microbiology Society, issn = 1466-5026, abstract= A novel Gram-stain-positive, aerobic bacterium, designated strain DHS C021T, was isolated from a limestone sample collected from the Puding Karst Ecosystem Research Station of Guizhou Province, southwest China. This strain developed branched vegetative mycelia, and its aerial mycelia fragmented into rod-shaped spores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised galactose, ribose and mannose. The respiratory quinone was identified as menaquinone MK-9(H4). The major cellular fatty acids were iso-C14 : 0 and iso-C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidyethanolamine, phosphatidylinositol, phosphotidylinositolmannosides and one unidentified phospholipid. The genomic DNA G+C content was 69.8 mol% and 16S rRNA gene sequence analysis showed that the strain belonged to the genus Lentzea and shared highest sequence similarity with Lentzea albida CGMCC 4.1727T (98.8 %) and Lentzea waywayandensis CGMCC 4.1646T (98.5 %). However, it could be distinguished from these reference strains based on the low levels of DNA–DNA relatedness (54.5±2.7 and 41.7±3.2 %, respectively). On the basis of morphological, chemotaxonomic and phylogenetic characteristics, and DNA–DNA hybridization data, strain DHS C021T represents a novel species of the genus Lentzea , for which the name Lentzea pudingi sp. nov. is proposed. The type strain is DHS C021T (=CGMCC 4.7319T=KCTC 39694T)., language=, type=