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- Volume 67, Issue 12
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f Streptococcus azizii sp. nov., isolated from naïve weanling mice
- Authors: Patricia Lynn Shewmaker1 , Anne M. Whitney1 , Christopher A. Gulvik1 , Neil S. Lipman2
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1 1Centers for Disease Control and Prevention, Atlanta, Georgia 30329, USA 2 2Center of Comparative Medicine and Pathology, Memorial Sloan Kettering Cancer Center and Weill Cornell Medicine, New York, NY 10065, USA
- *Correspondence: Patricia Lynn Shewmaker [email protected]
- First Published Online: 12 October 2017, International Journal of Systematic and Evolutionary Microbiology 67: 5032-5037, doi: 10.1099/ijsem.0.002407
- Subject: New Taxa - Firmicutes and Related Organisms
- Received:
- Accepted:
- Cover date:




Streptococcus azizii sp. nov., isolated from naïve weanling mice, Page 1 of 1
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Three isolates of a previously reported novel catalase-negative, Gram-stain-positive, coccoid, alpha-haemolytic, Streptococcus species that were associated with meningoencephalitis in naïve weanling mice were further evaluated to confirm their taxonomic status and to determine additional phenotypic and molecular characteristics. Comparative 16S rRNA gene sequence analysis showed nearly identical intra-species sequence similarity (≥99.9 %), and revealed the closest phylogenetically related species, Streptococcus acidominimus and Streptococcuscuniculi, with 97.0 and 97.5 % sequence similarity, respectively. The rpoB, sodA and recN genes were identical for the three isolates and were 87.6, 85.7 and 82.5 % similar to S. acidominimus and 89.7, 86.2 and 80.7 % similar to S. cuniculi , respectively. In silico DNA–DNA hybridization analyses of mouse isolate 12-5202T against S. acidominimus CCUG 27296T and S. cuniculi CCUG 65085T produced estimated values of 26.4 and 25.7 % relatedness, and the calculated average nucleotide identity values were 81.9 and 81.7, respectively. These data confirm the taxonomic status of 12-5202T as a distinct Streptococcus species, and we formally propose the type strain, Streptococcus azizii 12-5202T (=CCUG 69378T=DSM 103678T). The genome of Streptococcus azizii sp. nov. 12-5202T contains 2062 total genes with a size of 2.34 Mbp, and an average G+C content of 42.76 mol%.
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The GenBank/EMBL/DDBJ accession numbers for the whole-genome sequences of S. azizii 12-5202T, 12-5291, 13-1151-1, and S. acidominimus CCUG 27296T and S. cuniculi 65085T are MSPR00000000, MSPT00000000, MSPS00000000, MSJL00000000 and MSJM00000000, respectively. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, rpoB, sodA and recN genes are BVE84_10155, BVE84_07760, BVE84_10065 and BVE84_01660, respectively.
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Two supplementary tables and three supplementary figures are available with the online version of this article.
- Keyword(s): taxonomy, firmicutes, Streptococcus, mice
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