@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002425, author = "Ngaemthao, Wipaporn and Pujchakarn, Tawanmol and Chunhametha, Suwanee and Suriyachadkun, Chanwit", title = "Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "12", pages = "5114-5119", doi = "https://doi.org/10.1099/ijsem.0.002425", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002425", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "endophytic actinomycetes", keywords = "Verrucosispora endophytica sp. nov.", abstract = "A novel endophytic filamentous bacterium strain A-T 7972T, was isolated from wild orchid Grosourdya appendiculata (Blume) Rchb.f. collected in Thailand. The bacterium developed single non-motile spores with warty surface on substrate mycelia. The taxonomic position was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 7972T belonged to the genus Verrucosispora and shared the highest sequence similarity with V. lutea YIM 013T (98.71 %) and V. gifhornensis DSM 44337T (98.53 %). The values of DNA–DNA relatedness that distinguished this novel strain from its closest species were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were mannose, ribose, glucose and xylose. The predominant menaquinone was MK-9(H4). The predominant fatty acids were branched fatty acids iso-C15 : 0 and iso-C16 : 0. The diagnostic phospholipids profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 70.5 mol%. Based on its phenotypic, chemotaxonomic and genotypic characteristics, the new isolate A-T 7972T (=BCC 50981T=TBRC 6031T=NBRC 112512T) is proposed to be the type strain of novel species, Verrucosispora endophytica sp. nov.", }