1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 12

sp. nov., an agar-degrading bacterium isolated from marine alga Ueda Free

Abstract

A novel Gram-stain-negative, aerobic, rod-shaped, non-flagellated and agar-digesting marine bacterium, designated as HZ1, was isolated from the marine alga Ueda (AST58-103) collected from the coastal area of Weihai, PR China. Phylogenetic analysis based on 16S rRNA gene sequences placed HZ1 in the genus , and it formed a distinct clade in the phylogenetic tree with the type strains of and , with 97.0 % and 96.7 % 16S rRNA gene sequence similarities, respectively. The DNA G+C content of the isolate was 31.8 mol%. HZ1 contained MK-6 as the predominant menaquinone and iso-C, summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and iso-CG as the major fatty acids. The major polar lipids were phosphatidylethanolamine, four unidentified lipids and five unidentified aminolipids. On the basis of the results of the phylogenetic analysis and phenotypic properties, it is concluded that HZ1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HZ1 (=MCCC 1H00174=KCTC 52476).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , agar degradation , polyphasic characterization and Tenacibaculum
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002432
2017-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/12/5139.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002432&mimeType=html&fmt=ahah

References

  1. Yun EJ, Lee S, Kim HT, Pelton JG, Kim S et al. The novel catabolic pathway of 3,6-anhydro-l-galactose, the main component of red macroalgae, in a marine bacterium. Environ Microbiol 2015; 17:1677–1688 [View Article][PubMed]
     [Google Scholar]
  2. Li DQ, Zhou YX, Liu T, Chen GJ, Du ZJ. Catenovulum maritimus sp. nov., a novel agarolytic gammaproteobacterium isolated from the marine alga Porphyra yezoensis Ueda (AST58-103), and emended description of the genus Catenovulum. Antonie van Leeuwenhoek 2015; 108:427–434 [View Article][PubMed]
     [Google Scholar]
  3. Zhou YX, Wang C, Du ZJ, Chen GJ. Aquimarina agarivorans sp. nov., a genome-sequenced member of the class Flavobacteriia isolated from Gelidium amansii. Int J Syst Evol Microbiol 2015; 65:2684–2688 [View Article][PubMed]
     [Google Scholar]
  4. Park S, Choi WC, Oh TK, Yoon JH. Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. Int J Syst Evol Microbiol 2011; 61:2573–2576 [View Article][PubMed]
     [Google Scholar]
  5. Oh YS, Kahng HY, Lee DH, Lee SB. Tenacibaculum jejuense sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 2012; 62:414–419 [View Article][PubMed]
     [Google Scholar]
  6. Xu ZX, Mu X, Zhang HX, Chen GJ, Du ZJ. Marinifilum albidiflavum sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2016; 66:4589–4593 [View Article][PubMed]
     [Google Scholar]
  7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  9. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  10. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  11. Dong XZ, Cai MY. Determination of Biochemical Properties. Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp. 378–398
     [Google Scholar]
  12. Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M et al. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense. Int J Syst Evol Microbiol 2007; 57:1095–1107 [View Article][PubMed]
     [Google Scholar]
  13. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  14. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [View Article][PubMed]
     [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  16. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
     [Google Scholar]
  17. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
     [Google Scholar]
  18. Frette L, Jørgensen NO, Irming H, Kroer N. Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 2004; 54:519–524 [View Article][PubMed]
     [Google Scholar]
  19. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 2001; 51:1639–1652 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002432
Loading
Tenacibaculum agarivorans sp. nov., an agar-degrading bacterium isolated from marine alga Porphyra yezoensis Ueda
Int J Syst Evol Microbiol 67, 5139 (2017); https://doi.org/10.1099/ijsem.0.002432
/content/journal/ijsem/10.1099/ijsem.0.002432
/content/journal/ijsem/10.1099/ijsem.0.002432
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error