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f Mangrovitalea sediminis gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from mangrove sediment

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  • Authors: Hu Liao1​ ,  Yuqian Li1​ ,  Xintong Guo1​ ,  Xiaolan Lin1​ ,  Qiliang Lai2​ ,  Hong Xu1​ ,  Tianling Zheng1​ , Yun Tian1​
    • VIEW AFFILIATIONS
    • 1​ 1​Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, PR China 2​ 2​Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China
  • *Correspondence: Yun Tian [email protected]
  • First Published Online: 18 October 2017, International Journal of Systematic and Evolutionary Microbiology 67: 5172-5178, doi: 10.1099/ijsem.0.002433
  • Subject: New Taxa - Proteobacteria
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  • A Gram-stain-negative, facultative anaerobic and oligotrophic, rod-shaped, and motile with single polar flagellum bacterial strain, designed M11-4 was isolated from mangrove sediment in Yunxiao Mangrove National Nature Reserve, China. Growth was observed at temperatures from 10 to 40 °C (optimum 30 °C), at salinities from 0.5 to 6 % (optimum 2–3 %), and at pH from 5 to 8 (optimum 6). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain M11-4 shared highest sequence similarity with the genus Marinobacter (92.5–95.0 %) and represented a distinct phylogenetic lineage in the family Alteromonadaceae . The G+C content of the genomic DNA was 58.2 mol%. The dominant fatty acids were C16 : 0, C16 : 0 N-alcohol, summed feature 9 (comprising iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The predominant respiratory quinone was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol; phosphatidylethanolamine; phosphatidylglycerol and an unidentified aminophospholipid. According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence analysis, the strain M11-4 should be assigned as a novel species of a novel genus for which the name Mangrovitalea sediminis gen. nov., sp. nov. is proposed. The type strain of Mangrovitalea sediminis is M11-4 (=MCCC 1K03312=JCM 32104).

  • The 16S rRNA gene sequence of strain M11-4 has been deposited in GenBank/EMBL/DDBJ under the accession number MF034046. The Whole Genome of strain M11-4 has been deposited at DDBJ/ENA/GenBank under the accession NTLB00000000.

  • Three supplementary figures are available with the online version of this article.

© 2017 IUMS | Published by the Microbiology Society

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2019-05-26
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