@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002444, author = "Shin, Su-Kyoung and Kim, Eunji and Yi, Hana", title = "Paenibacillus crassostreae sp. nov., isolated from the Pacific oyster Crassostrea gigas", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "58-63", doi = "https://doi.org/10.1099/ijsem.0.002444", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002444", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Paenibacillus crassostreae sp. nov.", keywords = "Paenibacillus", keywords = "Pacific oyster", keywords = "Crassostrea gigas", keywords = "LPB0068T", abstract = "A Gram-stain-positive, endospore-forming, rod-shaped, aerobic bacterium, designated LPB0068T, was isolated from a Pacific oyster (Crassostrea gigas) in Korea. This isolate was found to share the highest 16S rRNA gene sequence similarity with Paenibacillus macquariensis subsp. macquariensis DSM 2T (98.1 %) and Paenibacillus macquariensis subsp. defensor JCM 14954T (98.0 %). To establish the genomic relatedness of this isolate to its phylogenetic neighbours, its genome sequence and those of Paenibacillus antarcticus CECT 5836T, P. macquariensis subsp. macquariensis DSM 2T, P. macquariensis subsp. defensor JCM 14954T, and Paenibacillus glacialis DSM 22343T were determined. The low average nucleotide identity and digital DNA–DNA hybridization values exhibited by LPB0068T in relation to the other strains in this analysis revealed that it is distinct from other Paenibacillus species. The genome of strain LPB0068T consists of one chromosome and three circular plasmids, and had a DNA G+C content of 40.0 mol%. The major respiratory quinone was menaquinone-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified glycolipid, and two unidentified polar lipids. The major cellular fatty acids were anteiso-C15 : 0, C14 : 0, and C16 : 0. Based on genomic, phylogenetic, and phenotypic characteristics, this strain was clearly distinguished from other Paenibacillus species with validly published names and should therefore be classified as a novel species of the genus. The name Paenibacillus crassostreae sp. nov. is proposed, the type strain of which is LPB0068T (=KACC 18694T=JCM 31183T).", }