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f Nocardioides immobilis sp. nov., isolated from iron mine soil

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Nocardioides immobilis sp. nov., isolated from iron mine soil, Page 1 of 1

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  • A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521 was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521 belonged to genus Nocardioides in family Nocardioidaceae , with the highest sequence identity to Nocardioides panacisoli GSoil 346 (96.3 %). The DNA G+C content of strain FLL521 was 70.3 mol%. The cell-wall peptidoglycan type was ll-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the predominant fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The polar lipids of strain FLL521 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and three unidentified phospholipids. Strain FLL521 showed physiological and biochemical differences with the related strains in the Voges-Proskauer test, hydrolysis of l-arginine, utilization of d-mannose, inositol, phenylalanine and acid production from l-arabinose. Based on the phenotypic, genotypic and phylogenetic analyses, strain FLL521 represents a novel species of Nocardioides , for which the name Nocardioides immobilis sp. nov. is proposed. The type strain is FLL521 (=KCTC 39931=CCTCC AB 2017083).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Nocardioides immobilis FLL521 is KY684256.

  • One supplementary table and four supplementary figures are available with the online version of this article.

© 2017 IUMS | Published by the Microbiology Society

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/content/journal/ijsem/10.1099/ijsem.0.002450
2017-10-25
2019-02-15
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