@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002450, author = "Lu, Lingfei and Cao, Min and Wang, Dan and Yuan, Kai and Zhuang, Weiping and Guo, Wei and Wang, Gejiao", title = "Nocardioides immobilis sp. nov., isolated from iron mine soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "12", pages = "5230-5234", doi = "https://doi.org/10.1099/ijsem.0.002450", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002450", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "novel species", keywords = "Nocardioides immobilis", abstract = "A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521T was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521T belonged to genus Nocardioides in family Nocardioidaceae , with the highest sequence identity to Nocardioides panacisoli GSoil 346T (96.3 %). The DNA G+C content of strain FLL521T was 70.3 mol%. The cell-wall peptidoglycan type was ll-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the predominant fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The polar lipids of strain FLL521T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and three unidentified phospholipids. Strain FLL521T showed physiological and biochemical differences with the related strains in the Voges-Proskauer test, hydrolysis of l-arginine, utilization of d-mannose, inositol, phenylalanine and acid production from l-arabinose. Based on the phenotypic, genotypic and phylogenetic analyses, strain FLL521T represents a novel species of Nocardioides , for which the name Nocardioides immobilis sp. nov. is proposed. The type strain is FLL521T (=KCTC 39931T=CCTCC AB 2017083T).", }