RT Journal Article SR Electronic(1) A1 Lu, Lingfei A1 Cao, Min A1 Wang, Dan A1 Yuan, Kai A1 Zhuang, Weiping A1 Guo, Wei A1 Wang, GejiaoYR 2017 T1 Nocardioides immobilis sp. nov., isolated from iron mine soil JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 12 SP 5230 OP 5234 DO https://doi.org/10.1099/ijsem.0.002450 PB Microbiology Society, SN 1466-5034, AB A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521T was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521T belonged to genus Nocardioides in family Nocardioidaceae , with the highest sequence identity to Nocardioides panacisoli GSoil 346T (96.3 %). The DNA G+C content of strain FLL521T was 70.3 mol%. The cell-wall peptidoglycan type was ll-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the predominant fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The polar lipids of strain FLL521T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and three unidentified phospholipids. Strain FLL521T showed physiological and biochemical differences with the related strains in the Voges-Proskauer test, hydrolysis of l-arginine, utilization of d-mannose, inositol, phenylalanine and acid production from l-arabinose. Based on the phenotypic, genotypic and phylogenetic analyses, strain FLL521T represents a novel species of Nocardioides , for which the name Nocardioides immobilis sp. nov. is proposed. The type strain is FLL521T (=KCTC 39931T=CCTCC AB 2017083T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002450