@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002452, author = "Dahal, Ram Hari and Chaudhary, Dhiraj Kumar and Kim, Jaisoo", title = "Rurimicrobium arvi gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from farmland soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "12", pages = "5235-5243", doi = "https://doi.org/10.1099/ijsem.0.002452", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002452", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Chitinophagaceae", keywords = "sp. nov.", keywords = "farmland soil", keywords = "gen. nov", keywords = "Rurimicrobium arvi", abstract = "Two strains of bacteria, designated J107-1T and J107-2, were isolated from farmland soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, coccoid rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Both strains hydrolysed casein and tyrosine. Strains could grow at pH 9.0 and at 42 °C. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strains J107-1T and J107-2 formed a lineage within the phylum Bacteroidetes that was distinct from various members of the family Chitinophagaceae , including Ferruginibacter paludis HME8881T (89.71 % sequence similarity), Ferruginibacter yonginensis HME8442T, Niastella yeongjuensis GR20-13T (89.70 %), Chitinophaga niabensis DSM 24787T (89.57 %), Lacibacter cauensis CGMCC 1.7271T (89.56 %) and Taibaiella koreensis THG-DT86T (89.52 %). The 16S rRNA gene similarities of other members of the family Chitinophagaceae are less than 89.51 %. The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C17 : 0. The DNA G+C content of strains ranged from 47.8 to 48.0 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strains J107-1T and J107-2 represent a novel genus in the family Chitinophagaceae, for which the name Rurimicrobium arvi gen. nov., sp. nov. is proposed. The type strain of Rurimicrobium arvi is J107-1T (=KEMB 9005-549T=KACC 19167T=JCM 31921T).", }