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f Paenibacillus xanthanilyticus sp. nov., a xanthan-degrading bacterium isolated from soil

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  • Authors: Simin Ashraf1​ ,  Mohammad Reza Soudi1​ ,  Mohammad Ali Amoozegar2​ ,  Mahdi Moshtaghi Nikou3​ , Cathrin Spröer4​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran 2​ 2​Department of Microbiology, Extremophiles Laboratory, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran 3​ 3​Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran 4​ 4​Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany
  • *Correspondence: Mohammad Reza Soudi [email protected] [email protected]
  • First Published Online: 14 November 2017, International Journal of Systematic and Evolutionary Microbiology 68: 76-80, doi: 10.1099/ijsem.0.002453
  • Subject: New Taxa - Firmicutes and Related Organisms
  • Received:
  • Accepted:
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Paenibacillus xanthanilyticus sp. nov., a xanthan-degrading bacterium isolated from soil, Page 1 of 1

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  • A xanthan-degrading bacterium, strain AS7, was isolated from soil and its taxonomic position was determined using a polyphasic approach. Strain AS7 was a Gram-stain-variable, spore-forming, motile, aerobic, rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that strain AS7 belongs to the genus Paenibacillus , sharing the highest level of sequence similarity with Paenibacillus phyllosphaerae PALXIL04 (98.0 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. MK-7 was the dominant isoprenoid quinone and the major fatty acid was anteiso-C15 : 0. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unknown phospholipids. These chemotaxonomic characteristics were consistent with the isolate belonging to the genus Paenibacillus . The G+C content of the genomic DNA was 51.0 mol% and the DNA–DNA hybridization value between strain AS7 and P. phyllosphaerae PALXIL04 was only 14.4±2.5 %. On the basis of phylogenetic analyses, phenotypic and chemotaxonomic characteristics, and DNA–DNA relatedness value, strain AS7 represents a novel species of the genus Paenibacillus , for which the name Paenibacillus xanthanilyticus sp. nov. is proposed. The type strain is AS7 (=IBRC M 10987=LMG 29451).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AS7 is KT429627.

  • One supplementary figure is available with the online version of this article.

© 2018 IUMS | Published by the Microbiology Society

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