@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002459, author = "Fan, Miao-Chun and Nan, Li-Jun and Zhu, Ya-Min and Chen, Wei-Min and Wei, Ge-Hong and Lin, Yan-Bing", title = "Mitsuaria noduli sp. nov., isolated from the root nodules of Robinia pseudoacacia in a lead–zinc mine", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "87-92", doi = "https://doi.org/10.1099/ijsem.0.002459", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002459", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Mitsuaria noduli", keywords = "polyphasic taxonomy", keywords = "16S rRNA gene sequence", abstract = "A novel endophytic bacterium, designated strain HZ7T, was isolated from the root nodules of Robinia pseudoacacia growing in a lead–zinc mine in Mianxian County, Shaanxi Province, China. Cells were Gram-reaction-negative, aerobic, motile, rod-shaped, methyl-red-negative, catalase-positive, positive for chitosan-degrading activity and did not produce H2S. Strain HZ7T grew at 4–45 °C (optimum 25–30 °C), at pH 5–9 (optimum pH 7–8) and with 0–1 % (w/v) NaCl. The quinone type was ubiquinone 8 (UQ-8). The major fatty acids were identified as C16 : 0, C17 : 0 cyclo and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The G+C content of the genomic DNA was 68.5 mol% by whole genome sequencing. According to 16S rRNA gene sequence analysis, the closest phylogenetic relative was Mitsuaria chitosanitabida 3001T (99.05 % similarity). Genome relatedness was computed using average nucleotide identity and genome-to-genome distance analysis, both of which strongly supported strain HZ7Tas belonging to the genus Mitsuaria as a representative of a novel species. On the basis of phylogenetic analysis, chemotaxonomic data and physiological characteristics, strain HZ7T represents a novel species of the genus Mitsuaria , for which the name Mitsuaria noduli sp. nov. is proposed. The type strain is HZ7T (=JCM 31671T=CCTCC AB 2014353T).", }