RT Journal Article SR Electronic(1) A1 Dai, Yu-Mei A1 Zhang, Ling-Li A1 Li, Yan A1 Li, Yu-Qian A1 Deng, Xiao-Hui A1 Wang, Ting-Ting A1 Yang, Feng A1 Tian, Yong-Qiang A1 Li, Na A1 Zhou, Xing-Mei A1 Liu, Xiao-Feng A1 Li, Wen-JunYR 2018 T1 Characterization of Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov., isolated from a high-elevation wetland, by phenotypic and genomic analyses JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 1 SP 99 OP 105 DO https://doi.org/10.1099/ijsem.0.002464 PB Microbiology Society, SN 1466-5034, AB Two psychrotolerant facultative anaerobes, strains B7-2T and B5T, were isolated from the Zoige Wetland on the Qinghai-Tibetan Plateau. The 16S rRNA gene sequences of strains B7-2T and B5T shared high similarity (>99 %) with those of the type strains of the genus Trichococcus, while their digital DNA–DNA hybridization values with each other (49 %) and with the reference type strains (48–23 %) were lower than 70 %, which suggest that they represent two novel species of the genus Trichococcus. Cells of strains B7-2T and B5T were immotile cocci, grew in the temperature range of 4–37 °C (optimum 25 °C) and were alkaliphilic with optimum growth at pH 9.0. The major components of the cellular fatty acids were C16 : 0, anteiso-C17 : 0 and C18 : 0 for strain B7-2T, and C16 : 0, anteiso-C17 : 0, C18 : 1ω9c and C18 : 0 for strain B5T. The genomic DNA G+C contents were 46.0 and 46.7 mol% for strains B7-2T and B5T, respectively. Based on physiological and genomic characteristics, it is suggested that strains B7-2T and B5T represent two novel species within the genus Trichococcus, for which the names Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov. are proposed. The type strains are B7-2T (=DSM 104691T=KCTC 33886T) and B5T (=DSM 104692T=KCTC 33885T), respectively., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002464