- Home
- Publications
- International Journal of Systematic and Evolutionary Microbiology
- Volume 68, Issue 1
- Article

f Pseudogracilibacillus endophyticus sp. nov., a moderately thermophilic and halophilic species isolated from plant root
- Authors: Jisun Park1 , Min-Kyeong Kim1 , Bo-Ram Yun1 , Ji-Hye Han2 , Seung Bum Kim1
-
- VIEW AFFILIATIONS
-
1 1Department of Microbiology and Molecular Biology, Chungnam National University, 99 Daehak-Ro, Yuseong, Daejeon 34134, Republic of Korea 2 2Bacterial Resources Research Team, Freshwater Bioresources Research Division, Nakdonggang National Institute of Biological Resources, Republic of Korea
- *Correspondence: Seung Bum Kim [email protected]
- First Published Online: 10 November 2017, International Journal of Systematic and Evolutionary Microbiology 68: 165-169, doi: 10.1099/ijsem.0.002475
- Subject: New Taxa - Firmicutes and Related Organisms
- Received:
- Accepted:
- Cover date:




Pseudogracilibacillus endophyticus sp. nov., a moderately thermophilic and halophilic species isolated from plant root, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/ijsem/68/1/165_ijsem002475-1.gif
-
A Gram-stain-positive strain, designated DT7-02T, was isolated from the surface-sterilized root of Oenotherabiennis (evening primrose) and subjected to taxonomic characterization. Cells of DT7-02T were slender rod-shaped, motile by means of flagella, and oxidase- and catalase-positive. The colonies were circular, pinkish-yellow, opaque, glistering and 1–2 mm in diameter. The strain was moderately thermophilic and halophilic, as growth occurred at 20–44 °C (optimum 40 °C), pH 7–10 (optimum pH 8–9) and in the presence of 0–8 % of NaCl (optimum 4 %) in tryptic soy broth. The analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus Pseudogracilibacillus of the family Bacillaceae , and the sequence similarity was 96.5 % with Pseudogracilibacillus auburnensis P-207T and 95.9 % with Pseudogracilibacillus marinus NIOT-bflm-S4T. Other related taxa were Ornithinibacillus contaminans DSM 22953T and Sinibacillus soli KCTC 33117T, with 16S rRNA gene sequence similarities of 95.4 and 94.3 %, respectively. The major cellular fatty acids of DT7-02T were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 35.1 mol%, and the respiratory quinone was MK-7. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The combination of chemotaxonomic properties enabled differentiation of DT7-02T from the other two species of the genus Pseudogracilibacillus . The results of phylogenetic, phenotypic and chemotaxonomic analyses demonstrate that strain DT7-02T (=KCTC 33854T=JCM 31192T) merits recognition as representing a novel species of the genus Pseudogracilibacillus , for which the name Pseudogracilibacillus endophyticus sp. nov. is proposed.
-
The nucleotide accession number for the 16S rRNA gene sequence of strain DT7-02T is KM253061.
-
Two supplementary tables and two supplementary figures are available with the online version of this article.
- Keyword(s): halophile, moderate thermophile, endophyte, Bacillaceae, Pseudogracilibacillus endophyticus
© 2018 IUMS | Published by the Microbiology Society
-
1. Glaeser SP, McInroy JA, Busse HJ, Kämpfer P. Pseudogracilibacillus auburnensis gen. nov., sp. nov., isolated from the rhizosphere of Zea mays. Int J Syst Evol Microbiol 2014; 64: 2442– 2448 [CrossRef] [PubMed]
-
2. Verma P, Saravanan N, Jia B, Jeon CO, Dharani G et al. Pseudogracilibacillus marinus sp. nov., isolated from a biofilm formed in coastal seawater. Int J Syst Evol Microbiol 2016; 66: 3443– 3448 [CrossRef] [PubMed]
-
3. Hardoim PR, van Overbeek LS, Elsas JD. Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 2008; 16: 463– 471 [CrossRef] [PubMed]
-
4. Saunders M, Glenn AE, Kohn LM. Exploring the evolutionary ecology of fungal endophytes in agricultural systems: using functional traits to reveal mechanisms in community processes. Evol Appl 2010; 3: 525– 537 [CrossRef] [PubMed]
-
5. Kuldau G, Bacon C. Clavicipitaceous endophytes: their ability to enhance resistance of grasses to multiple stresses. Biological Control 2008; 46: 57– 71 [CrossRef]
-
6. Zabalgogeazcoa I. Fungal endophytes and their interaction with plant pathogens: a review. Spanish J Agric Res 2008; 6: 138– 146 [CrossRef]
-
7. Strobel GA, Knighton B, Kluck K, Ren Y, Livinghouse T et al. The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072). Microbiology 2008; 154: 3319– 3328 [CrossRef] [PubMed]
-
8. Kim TS, Han JH, Joung Y, Kim SB. Paenibacillus oenotherae sp. nov. and Paenibacillus hemerocallicola sp. nov., isolated from the roots of herbaceous plants. Int J Syst Evol Microbiol 2015; 65: 2717– 2725 [CrossRef] [PubMed]
-
9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
-
10. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
-
11. Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evo 1987; 4: 406– 425
-
12. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969; pp. 21– 132 [Crossref]
-
13. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Biol 1969; 18: 1– 32 [CrossRef]
-
14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
-
15. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64: 689– 691 [CrossRef] [PubMed]
-
16. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef] [PubMed]
-
17. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64: 346– 351 [CrossRef] [PubMed]
-
18. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
-
19. Schaeffer AB, Fulton MD. A simplified method of staining endospores. Science 1933; 77: 194 [CrossRef] [PubMed]
-
20. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178: 703 [CrossRef] [PubMed]
-
21. Pérez-Miranda S, Cabirol N, George-Téllez R, Zamudio-Rivera LS, Fernández FJ. O-CAS, a fast and universal method for siderophore detection. J Microbiol Methods 2007; 70: 127– 131 [CrossRef] [PubMed]
-
22. Park J, Kim YR, Kim MK, Jo JH, Im WT et al. Humibacter soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 2016; 66: 2509– 2514 [CrossRef] [PubMed]
-
23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990

Supplementary Data
Data loading....

Article metrics loading...

Full text loading...
Author and Article Information
-
This Journal
/content/journal/ijsem/10.1099/ijsem.0.002475dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/ijsem AND -contentType:BlogPost104 -
Other Society Journals
/content/journal/ijsem/10.1099/ijsem.0.002475dcterms_title,dcterms_subject-pub_serialIdent:journal/ijsem AND -contentType:BlogPost104 -
PubMed
-
Google Scholar
Figure data loading....