@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002506, author = "Kristyanto, Sylvia and Chaudhary, Dhiraj Kumar and Kim, Jaisoo", title = "Stakelama algicida sp. nov., novel algicidal species of the family Sphingomonadaceae isolated from seawater", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "317-323", doi = "https://doi.org/10.1099/ijsem.0.002506", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002506", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "seawater", keywords = "Stakelama algicida sp. nov.", keywords = "Sphingomonadaceae", keywords = "South Sea", keywords = "algalytic activity", abstract = "We conducted a taxonomic study of two algicidal bacteria, designated strains Yeonmyeong 1-13T and Yeonmyeong 1-11, isolated from seawater off Geoje Island in the South Sea, Republic of Korea. The two novel strains were yellow-pigmented, halotolerant, Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped bacteria. Both strains were able to grow at 5–39 °C, pH 5.0–10.0 and 0–11 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strains Yeonmyeong 1-13T and Yeonmyeong 1-11 belonged to the genus Stakelama and are closely related to Stakelama pacifica JLT832T (98.37% and 98.22 % sequence similarity, respectively). The pairwise sequence similarity between strains Yeonmyeong 1-13T and Yeonmyeong 1-11 was observed to be 99.50 %. In both strains, the only respiratory quinone was ubiquinone-10; the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the major fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0 2-OH. DNA G+C content values of strains Yeonmyeong 1-13T and Yeonmyeong 1-11 were 65.1% and 64.9 mol%, respectively. The DNA–DNA relatedness between Yeonmyeong 1-13T and S. pacifica DSM 25059T was 28.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain Yeonmyeong 1-13T from its closest phylogenetic neighbours. Thus, strains Yeonmyeong 1-13T and Yeonmyeong 1-11 represent a novel species of the genus Stakelama , for which the name Stakelama algicida sp. nov. is proposed. The type strain is Yeonmyeong 1-13T (=KEMB 9005-324T=JCM 31498T).", }