RT Journal Article SR Electronic(1) A1 Kristyanto, Sylvia A1 Chaudhary, Dhiraj Kumar A1 Kim, JaisooYR 2018 T1 Stakelama algicida sp. nov., novel algicidal species of the family Sphingomonadaceae isolated from seawater JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 1 SP 317 OP 323 DO https://doi.org/10.1099/ijsem.0.002506 PB Microbiology Society, SN 1466-5034, AB We conducted a taxonomic study of two algicidal bacteria, designated strains Yeonmyeong 1-13T and Yeonmyeong 1-11, isolated from seawater off Geoje Island in the South Sea, Republic of Korea. The two novel strains were yellow-pigmented, halotolerant, Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped bacteria. Both strains were able to grow at 5–39 °C, pH 5.0–10.0 and 0–11 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strains Yeonmyeong 1-13T and Yeonmyeong 1-11 belonged to the genus Stakelama and are closely related to Stakelama pacifica JLT832T (98.37% and 98.22 % sequence similarity, respectively). The pairwise sequence similarity between strains Yeonmyeong 1-13T and Yeonmyeong 1-11 was observed to be 99.50 %. In both strains, the only respiratory quinone was ubiquinone-10; the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the major fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0 2-OH. DNA G+C content values of strains Yeonmyeong 1-13T and Yeonmyeong 1-11 were 65.1% and 64.9 mol%, respectively. The DNA–DNA relatedness between Yeonmyeong 1-13T and S. pacifica DSM 25059T was 28.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain Yeonmyeong 1-13T from its closest phylogenetic neighbours. Thus, strains Yeonmyeong 1-13T and Yeonmyeong 1-11 represent a novel species of the genus Stakelama , for which the name Stakelama algicida sp. nov. is proposed. The type strain is Yeonmyeong 1-13T (=KEMB 9005-324T=JCM 31498T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002506