@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002514, author = "Chaudhary, Dhiraj Kumar and Kim, Jaisoo", title = "Sphingopyxis nepalensis sp. nov., isolated from oil-contaminated soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "364-370", doi = "https://doi.org/10.1099/ijsem.0.002514", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002514", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "oil-degrading bacteria", keywords = "Sphingopyxis nepalensis sp. nov.", keywords = "Proteobacteria", keywords = "Sphingomonadaceae", keywords = "oil-contaminated soil", abstract = "During a study of oil-degrading bacteria, three yellow-coloured, Gram-stain-negative, non-motile and rod-shaped bacteria, designated strains Ktm-14T, Ktm-17 and Ktm-18, were isolated from oil-contaminated soil of Biratnagar, Morang, Nepal. The strains were able to grow at 15–37 °C, pH 4.5–10.0 and 0–2 % (w/v) NaCl concentration. Strains Ktm-14T, Ktm-17 and Ktm-18 were characterized by multiple taxonomic approaches. Based on 16S rRNA gene sequence analysis, strains Ktm-14T, Ktm-17 and Ktm-18 belonged to the genus Sphingopyxis and shared highest sequence similarity with Sphingopyxis ginsengisoli Gsoil 250T (98.94 %). The only respiratory quinone was ubiquinone-10 and the predominant polyamine was spermidine. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and sphingoglycolipids. The predominant fatty acids were C17 : 1ω6c, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The DNA G+C content values of strains Ktm-14T, Ktm-17 and Ktm-18 were 65.8, 65.9 and 65.6 mol%, respectively. The DNA–DNA relatedness between Ktm-14T and Ktm-17 and Ktm-18 were higher than 70 % but with closely related reference strains were less than 40 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain Ktm-14T from its closest phylogenetic neighbours. Thus, strain Ktm-14T represents a novel species of the genus Sphingopyxis , for which the name Sphingopyxis nepalensis sp. nov. is proposed. The type strain is Ktm-14T (=KEMB 9005-694T=KACC 19389T=JCM 32250T), and strains Ktm-17 and Ktm-18 represent two additional strains.", }