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Volume 68, Issue 1

sp. nov., a novel member of the genus isolated from the river Ganges, India Free

Abstract

A bacterial strain, designated ASS-1, was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were Cω7/ 16 : 1 Cω6, Cω7 and C. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1 showed high similarity to CC-G9A (98.2 %), ATCC 14909 (98.2 %), DSM 1045 (98.1 %), IPL-1 (98.1 %) and HT-3 (98.0 %). Analysis of its and housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93 % with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, genes and the whole genome assigned it to the genus The results of digital DNA–DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95 %, respectively, for species delineation). Strain ASS-1 also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42 °C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1 should be regarded as a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ASS-1 (=KCTC 52437=CCM 8778).

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Keyword(s): 16S rRNA , ANI , GGDC , P. guguanensis , Pseudomonas fluvialis and rpoB
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2018-01-01
2024-04-16
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Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India
Int J Syst Evol Microbiol 68, 402 (2018); https://doi.org/10.1099/ijsem.0.002520
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