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Volume 68, Issue 1

sp. nov., a novel cellulolytic bacterium isolated from stream bank soil Free

Abstract

Two isolates of bacterial strains A15 and A17 were isolated from stream bank soil in Kyonggi University. Cells were aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, non-spore-forming, rod-shaped, opaque, and cream coloured. Both strains hydrolysed CM-cellulose. Strains were able to grow at 20–42 °C, pH 5.5–10.0 and at 1.5 % NaCl concentration (w/v). Indole test was positive. Analyses of phylogenetic trees based on its 16S rRNA gene sequences indicated that strain A15 formed a lineage within the family of the phylum which was distinct from S3 (98.4 % sequence similarity) and LM-6 (97.4 %). The sole detected respiratory quinone was Q–10. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The major cellular fatty acids were C cyclo8, C, summed feature 8 (Cω7 and/or Cω6), Ccyclo and C. The DNA G+C contents of strains A15 and A17 were 63.4 and 62.9 mol%, respectively. DNA–DNA relatedness between strain A15 and other two members of the genus ranged from 25 to 37 %. The polyphasic characterization revealed strains A15 and A17 represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is A15 (=KEMB 9005-522=KACC 19102=NBRC 112682).

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Keyword(s): Alphaproteobacteria , cellulolytic , Ferrovibrio soli sp. nov. , Rhodospirillaceae and stream bank soil
© 2018 IUMS
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2018-01-01
2024-04-20
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Ferrovibrio soli sp. nov., a novel cellulolytic bacterium isolated from stream bank soil
Int J Syst Evol Microbiol 68, 427 (2018); https://doi.org/10.1099/ijsem.0.002527
/content/journal/ijsem/10.1099/ijsem.0.002527
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