%0 Journal Article %A Wang, Cong %A Huang, Yan %A Li, Li %A Guo, Jun %A Wu, Zhengyun %A Deng, Yu %A Dai, Lirong %A Ma, Shichun %T Lactobacillus panisapium sp. nov., from honeybee Apis cerana bee bread %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 3 %P 703-708 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002538 %K Lactobacillus %K bee bread %K Apis cerana %K Lactobacillus panisapium %I Microbiology Society, %X A novel facultatively anaerobic, Gram-stain-positive, non-motile, non-spore-forming, catalase-negative bacterium of the genus Lactobacillus , designated strain Bb 2-3T, was isolated from bee bread of Apis cerana collected from a hive in Kunming, China. The strain was regular rod-shaped. Optimal growth occurred at 37 °C, pH 6.5 with 5.0 g l−1 NaCl. The predominant fatty acids were C18 : 1ω9c, C16 : 0 and C19 : 0 iso. Respiratory quinones were not detected. Seven glycolipids, three lipids, phosphatidylglycerol and diphosphatidylglycerol were detected. The peptidoglycan type A4α l-Lys-d-Asp was determined. Strain Bb 2-3T was closely related to Lactobacillus bombicola DSM 28793T, Lactobacillus apis LMG 26964T and Lactobacillus helsingborgensis DSM 26265T, with 97.8, 97.6 and 97.0 % 16S rRNA gene sequence similarity, respectively. A comparison of two housekeeping genes, rpoA and pheS, revealed that strain Bb 2-3T was well separated from the reference strains of species of the genus Lactobacillus . The average nucleotide identity between strain Bb 2-3T and the type strains of closely related species was lower than the 95–96 % threshold value for delineation of genomic prokaryotic species. The G+C content of the genomic DNA of strain Bb 2-3T was 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain Bb 2-3T is proposed to represent a novel species of the genus Lactobacillus , for which we propose the name Lactobacillus panisapium sp. nov. The type strain is Bb 2-3T (=DSM 102188T=ACCC 19955T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002538