RT Journal Article SR Electronic(1) A1 Yuki, Masahiro A1 Sakamoto, Mitsuo A1 Nishimura, Yuki A1 Ohkuma, MoriyaYR 2018 T1 Lactococcus reticulitermitis sp. nov., isolated from the gut of the subterranean termite Reticulitermes speratus JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 2 SP 596 OP 601 DO https://doi.org/10.1099/ijsem.0.002549 PB Microbiology Society, SN 1466-5034, AB Strain Rs-Y01T was isolated from the gut of the wood-feeding subterranean termite Reticulitermes speratus. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain Rs-Y01T belonged to the genus Lactococcus and was most closely related to Lactococcus raffinolactis JCM 5706T with 98.1 % similarity in the 16S rRNA gene, followed by Lactococcus piscium JCM 16647T (97.2 %). Genomic comparisons of strain Rs-Y01T with L. raffinolactis JCM 5706Twere made using the Genome-to-Genome Distance Calculator and average nucleotide identity analysis (values indicated 29.2 and 84.6 %, respectively). Strain Rs-Y01T was a Gram-stain-positive, facultatively anaerobic, non-motile coco-bacilli and formed l-lactic acid. The sugar fermentation and enzyme reactions of strain Rs-Y01T differed from those of other species of the genus Lactococcus . The major cellular fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c; 32.0 %), C16 : 0 (29.7 %) and C14 : 0 (18.1 %). Based on these characteristics, strain Rs-Y01T represents a novel species of the genus Lactococcus , for which the name Lactococcus reticulitermitis sp. nov. is proposed. The type strain is Rs-Y01T (=JCM 32106T=DSM 105715T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002549