1887

Abstract

A novel thermophilic actinomycete, designated strain 3-12X, was isolated from mushroom compost in Guangxi University, Nanning, China. The novel isolate contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were glucose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H). The polar phospholipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, ninhydrin-positive phosphoglycolipids and glycolipids. Major fatty acids were so-C and C. The G+C content of the genomic DNA was 74.6 %. The 16S rRNA gene sequence analysis showed that the closest phylogenetic neighbour of strain 3-12X was ATCC 43196 (97.0 %), other closely related strains all belonged to the family and showed more than 6 % divergence. The chemotaxonomic characteristics of strain 3-12X were significantly different from ATCC 43196 and DNA–DNA hybridization showed low relatedness (48.6–55.6 %) between them, so they should be different species. was removed from the genus by Zhang 1998 on the basis of phylogenetic, chemotaxonomic and phenotypic evidence, but its taxonomic position remains uncertain. Based on the phenotypic and phylogenetic data, strain 3-12X represents a novel species in a new genus in the family . The name gen. nov., sp. nov. is proposed. The type strain of is 3-12X (=DSM 46781=CGMCC 4.7160). We also propose transferring Zhang 1998 to comb. nov. (type strain ATCC 43196=JCM 6244).

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2018-02-01
2024-03-29
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