1887

Abstract

A cream-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain Brt-F, was isolated from oil-contaminated soil. Strain Brt-F was able to grow from 15 to 45 °C, pH 6.5–10.5 and 0–4.5 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain Brt-F represented a member of the genus and shared highest sequence similarity with DH-5 (99.4 %); H7 (95.7 %) and H-12 (94.3 %). The only respiratory quinone was menaquinone-7, the major polar lipid was phosphatidylethanolamine and the predominant fatty acids were summed feature 3 (Cω7 and/or Cω6), -C, and -C 3-OH. The DNA G+C content was 43.4 mol%. The DNA–DNA relatedness value between strain Brt-F and KCTC 42746 was 35.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain Brt-F represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Brt-F (=KEMB 9005-691=KACC 19392=JCM 32159).

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2018-02-01
2024-03-29
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