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Volume 68, Issue 2

sp. nov., isolated from yak milk Free

Abstract

A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C, iso-C 3-OH, iso-Cω9 and C 7 and/or Cω6 and/or iso-C 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C content of the strain was 38.9 mol%. The 16S rRNA gene sequence analysis indicated that strain YK2 was a member of the genus and closely related to and with pair-wise sequence similarity of 98.3 and 97.9 %, respectively. The sequence similarity to other members of the genus was between 92.6 to 96.3 %. Phylogenetic analysis showed that strain YK2 clustered with and together clustered with . DNA–DNA hybridization of strain YK2 with WCC 4521 and T5-12 showed a relatedness of only 38 and 54 %, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is YK2 (=MTCC 12631=KCTC 52685=JCM 31951).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Gram-negative , novel species , Sphingobacterium and yak milk
© 2018 IUMS
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2018-02-01
2024-04-19
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Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk
Int J Syst Evol Microbiol 68, 636 (2018); https://doi.org/10.1099/ijsem.0.002562
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