1887

Abstract

Two dark pink pigmented bacterial strains (M3 and M11) were isolated from crude oil contaminated desert sand from Kuwait. Both strains were Gram-stain-negative and small-rod to oval-shaped bacteria. Strains M3 and M11 grew at 13–42 °C (optimum, 30–35 °C) and pH 6.5–9.0 (optimum, 7.0–7.5). No additional NaCl was required for the growth of both strains. The genomic DNA G+C content of strains M3 and M11 were 69.5 and 69.0 mol%, respectively. Both strains were closely related and the mean DNA–DNA hybridization value was 92±1 %. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus . Strains M3 and M11 had a sequence similarity of 97.3 and 97.4 % with JC288, respectively. Both strains had <97 % 16S rRNA gene sequence similarity with other members of the genus . Strain M3 showed 18±2 and 13±2 % reassociation (based on DNA–DNA hybridization) with KCTC 42542 and KACC 11686, respectively. The major cellular fatty acids (>5 %) were identified as Cω6/Cω7, Cω6/Cω7 and C in both strains. Both strains showed diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidylcholine and unidentified glycolipid as major polar lipids. Based on distinct phenotypic, genotypic and phylogenetic differences from the previously described taxa, we propose the classification of strains M3 and M11 as representative of a novel species in the genus , for which the name sp. nov. is suggested. The type strain is M3 (=KEMB 2255-459=JCM 31275).

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2018-02-01
2024-03-28
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