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Volume 68, Issue 3

sp. nov., a novel bacterium isolated from cyanobacterial aggregates in a eutrophic lake Free

Abstract

A bacterial strain, designated TH057, was isolated from cyanobacterial aggregates in a eutrophic lake in China. Cells were observed to be slightly curved, rod-shaped, capsule-forming and stained Gram-negative. Optimal growth was obtained at pH 7.0 (range: pH 5–9) and 30 °C (range: 20–37 °C) in R2A broth. According to the absorption spectrum, carotenoids (455 and 490 nm) and light-harvesting complex LHI (857 nm) were present in the cells. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids were identified as Cω6, Cω7/Cω6, Cω6/Cω7 and C. The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid and two sphingoglycolipids. Strain TH057 shared highest 16S rRNA gene sequence similarity to so42 (96.8 %), followed by D40P (95.8 %). The genomic G+C content of strain TH057 was 66.1 mol% based on total genome calculations. The average nucleotide identity and the digital DNA–DNA hybridization value for the complete genomes were 81.0 and 23.0 % between strain TH057 and so42. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH057 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TH057 (=CGMCC 1.15803=LMG 30294).

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  • Accepted:
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Keyword(s): cyanobacterial aggregates and Sandarakinorhabdus cyanobacteriorum sp. nov.
© 2018 IUMS
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2018-03-01
2024-04-19
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References

  1. Gich F, Overmann J. Sandarakinorhabdus limnophila gen. nov., sp. nov., a novel bacteriochlorophyll a-containing, obligately aerobic bacterium isolated from freshwater lakes. Int J Syst Evol Microbiol 2006; 56:847–854 [View Article][PubMed]
     [Google Scholar]
  2. Kim M, Kang O, Zhang Y, Ren L, Chang X et al. Sphingoaurantiacus polygranulatus gen. nov., sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii . Int J Syst Evol Microbiol 2016; 66:91–100 [View Article][PubMed]
     [Google Scholar]
  3. Cai H, Jiang H, Krumholz LR, Yang Z. Bacterial community composition of size-fractioned aggregates within the phycosphere of cyanobacterial blooms in a eutrophic freshwater lake. PLoS One 2014; 9:e102879 [View Article][PubMed]
     [Google Scholar]
  4. Cai H, Yan Z, Wang A, Krumholz LR, Jiang H. Analysis of the attached microbial community on mucilaginous cyanobacterial aggregates in the eutrophic Lake Taihu reveals the importance of Planctomycetes . Microb Ecol 2013; 66:73–83 [View Article][PubMed]
     [Google Scholar]
  5. Cai H, Wang K, Huang S, Jiao N, Chen F. Distinct patterns of picocyanobacterial communities in winter and summer in the Chesapeake Bay. Appl Environ Microbiol 2010; 76:2955–2960 [View Article][PubMed]
     [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  7. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  9. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969 pp. 21–132 [Crossref]
     [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  11. Jia L, Feng X, Zheng Z, Han L, Hou X et al. Polymorphobacter fuscus sp. nov., isolated from permafrost soil, and emended description of the genus Polymorphobacter . Int J Syst Evol Microbiol 2015; 65:3920–3925 [View Article][PubMed]
     [Google Scholar]
  12. Chun J, Rainey FA. Integrating genomics into the taxonomy and systematics of the Bacteria and Archaea . Int J Syst Evol Microbiol 2014; 64:316–324 [View Article][PubMed]
     [Google Scholar]
  13. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  14. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
     [Google Scholar]
  15. Aziz RK, Bartels D, Best AA, Dejongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article][PubMed]
     [Google Scholar]
  16. Paisley R. MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual Newark, DE: MIDI; 1996
     [Google Scholar]
  17. Kates M. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986 pp. 106–107
     [Google Scholar]
  18. Collins MD. Isoprenoid quinone analysis in classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–287
     [Google Scholar]
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Sandarakinorhabdus cyanobacteriorum sp. nov., a novel bacterium isolated from cyanobacterial aggregates in a eutrophic lake
Int J Syst Evol Microbiol 68, 730 (2018); https://doi.org/10.1099/ijsem.0.002571
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