@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002578, author = "Ko, Yongseok and Yim, Jeeyeo and Hwang, Woon Mo and Kang, Keunsoo and Ahn, Tae-Young", title = "Roseomonas fluminis sp. nov. isolated from sediment of a shallow stream", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "3", pages = "782-787", doi = "https://doi.org/10.1099/ijsem.0.002578", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002578", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Roseomonas", keywords = "sediment", keywords = "Alphaproteobacteria", keywords = "Roseomonas fluminis", abstract = "An aerobic, Gram-negative, motile by means of a single polar flagellum, and ovoid-shaped bacterium, designated D3T, was isolated from shallow stream sediments in Sinan-gun, South Korea. Growth occurred at 15–40 °C (optimum 35 °C), at pH 7.0–8.0 (optimum pH 7.0), and at an optimum NaCl concentration of 0.5 % (w/v). The major cellular fatty acids (>7 % of the total) were C16 : 0, C18 : 0 2-OH, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant quinone was ubiquinone-10, and the G+C content of the genomic DNA of strain D3T was 73.1 mol%. The major polyamine was spermidine. The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D3T clustered with Roseomonas aquatica TR53T within the genus Roseomonas . The 16S rRNA gene sequence of strain D3T showed the highest sequence similarity to R. aquatica TR53T (95.9 %), followed by Roseomonas rosea 173-96T (95.7 %) and Roseomonas aerilata 5420S-30T (95.0 %). Based on the phenotypic, phylogenetic and chemotaxonomic characterization, strain D3T represents a novel species of the genus Roseomonas , for which the name Roseomonas fluminis sp. nov. is proposed. The type strain is D3T (=KACC 19269T=JCM 31968T).", }