@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002588, author = "Singh, Harjodh and Kaur, Manpreet and Kaur, Lakhwinder and Sharma, Shivani and Mishra, Sunita and Tanuku, Naga Radha Srinivas and Pinnaka, Anil Kumar", title = "Bacillus lacus sp. nov., isolated from a water sample of a salt lake in India", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "3", pages = "801-809", doi = "https://doi.org/10.1099/ijsem.0.002588", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002588", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "chemotaxonomy", keywords = "Bacillus", keywords = "16S rRNA gene sequence based phylogeny", abstract = "A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2 mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48 h growth at 37 °C with pH 9.0. Growth occurred at 25–42 °C, 0–4 % (w/v) NaCl and at a pH of 7–12. Strain AK74T was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C content of strain AK74T was 51.6 mol%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus niabensis , Bacillus idriensis and Bacillus halosaccharovorans were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5%, respectively. Phylogenetic analysis showed that strain AK74T clustered with Bacillus mangrove and together clustered with Bacillus idriensis and Bacillus indicus . Based on its phenotypic characteristics and on phylogenetic inference, strain AK74T represents a novel species of the genus Bacillus , for which the name Bacillus lacus sp. nov. is proposed. The type strain is AK74T (=MTCC 12638T=KCTC 33946T=JCM 32185T).", }