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Volume 68, Issue 3

Proposal of sp. nov., isolated from a marine sponge of the genus Free

Abstract

A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42, was isolated from a marine sponge of the genus , which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany. The strain grew at 4–34 °C (optimum 28 °C), in the presence of 0.5–9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5–10.0 (optimum pH 7.5). Strain MAA42 shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Sequence similarities to all other closely related type strains were below 97 %. DNA–DNA hybridization of strain MAA42 with DSM 22008 resulted in values of 4.7 % (reciprocal 17.7 %). Major cellular fatty acids of strain MAA42 were Cω7 (66.2 %), C 2-OH (17.4 %), and C (14.1 %). Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone. The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid. Glucuronopyranosyldiglyceride was present as a minor compound. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The genomic DNA G+C content was 52.8 mol%. Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42 represents a novel species of the genus for which the name is proposed. The type strain is MAA42 (=CCM 8709=CIP 111178=LMG 29765).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Litorimonas , marine microbe and sponge
© 2018 IUMS
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2018-03-01
2024-04-19
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Proposal of Litorimonas haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona
Int J Syst Evol Microbiol 68, 835 (2018); https://doi.org/10.1099/ijsem.0.002592
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