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Volume 68, Issue 3

A novel genus of the class , gen. nov., sp. nov., isolated from lake sediment Free

Abstract

A novel actinobacterial strain, designated X5, was isolated from the sediment of Taihu Lake in China and was subjected to a polyphasic taxonomic characterization. The strain formed orange–red colonies comprising aerobic, Gram-stain-negative, rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the organism was closely related to the genus and consistently formed a distinct clade along with the members of this genus. The closest phylogenetic neighbour was NBRC 12702 with 93.7 % 16S rRNA gene sequence similarity. The major fatty acids (>10 %) were iso-C (18.7 %), Cω9 (18.6 %) and Cω8 (14.0 %). The genomic DNA G+C content was 74.4 mol%. The organism contained menaquinone MK-8(H), MK-9(H) and an unidentified menaquinone. Polar lipids were composed of phosphatidylglycerol, an unidentified lipid, two unidentified phospholipids and two unidentified aminolipids. The whole-cell sugars contained ribose, xylose, mannose, glucose and galactose. The cell-wall peptidoglycan contained -diaminopimelic acid. Based on the physiological, biochemical and chemotaxonomic data, the organism is proposed to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is X5 (=CGMCC 4.7317=NBRC 112237).

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Keyword(s): lake sediment , Longivirga aurantiaca , polyphasic identification and taxonomic position
© 2018 IUMS
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2018-03-01
2024-04-19
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A novel genus of the class Actinobacteria, Longivirga aurantiaca gen. nov., sp. nov., isolated from lake sediment
Int J Syst Evol Microbiol 68, 942 (2018); https://doi.org/10.1099/ijsem.0.002615
/content/journal/ijsem/10.1099/ijsem.0.002615
/content/journal/ijsem/10.1099/ijsem.0.002615
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