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Volume 68, Issue 4

sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing Free

Abstract

The indigo-reducing, facultatively anaerobic and obligately alkaliphilic strains Bf-1, Bf-2 and Bf-4 were isolated from an indigo fermentation liquor used for dyeing, which uses [composted Polygonum indigo ( Lour.) leaves] as a basic ingredient and was obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence analyses indicated that the closest neighbours of strain Bf-1 are DSM 100413 (98.3 % 16S rRNA gene sequence similarity), DSM 25386 (98.2 %) and LMG 28275 (97.7 %). The 16S rRNA gene sequence of strain Bf-1 was almost identical to the sequences of strains Bf-2 and Bf-4 (99.9 %). Cells of strain Bf-1 stained Gram-positive and formed straight rods that achieved motility through a pair of subpolar flagella. Strain Bf-1 grew at temperatures of between 15 and 45 °C with optimum growth at 33‒40 °C. The strain grew in the pH range of pH 8‒12, with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 41.7 %. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C and iso-C. Phylogenetically related neighbours, although demonstrating high 16S rRNA gene sequence similarity (>97.6 %) with strain Bf-1, exhibited less than 9 % relatedness in DNA–DNA hybridization experiments. Based on evidence from this polyphasic study, the isolates represent a novel species, for which the name sp. nov. is proposed. The type strain of this species is Bf-1 (=JCM 31807=NCIMB 15079).

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Keyword(s): alkaliphilic , Bacillus fermenti , indigo fermentation liquor and indigo reduction
© 2018 IUMS
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2018-04-01
2024-04-19
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Bacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing
Int J Syst Evol Microbiol 68, 1123 (2018); https://doi.org/10.1099/ijsem.0.002636
/content/journal/ijsem/10.1099/ijsem.0.002636
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