1887

Abstract

Strain 2CBEGH3, which is an obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-positive coccobacillus, was isolated from a faecal sample of a healthy Japanese man. The 16S rRNA gene sequence analysis showed that strain 2CBEGH3 represented a member of the family and formed a monophyletic cluster with DSM 7084 (93.6 % sequence similarity), strain lac31 (93.0 %), JCM 14553 (92.7 %) and strain SK9K4 (92.7 %) as closest neighbours and species. The gene sequence analysis supported the phylogenetic relationships based on the 16S rRNA gene sequences, with sequence similarity values of 82.1–84.7 % to the four species described above. A unique three-base (one amino acid residue) insertion was found in the alignment regions of the gene sequence of strain 2CBEGH3. The major end products from -glucose were - and -lactic acids produced at the ratio of 75 : 25, while four species of the genus produced - and -lactic acids at ratios of 94–98 : 2–6. The isolate formed characteristic crater-like colonies on Eggerth–Gagnon agar plates. The major cellular fatty acids were Cω9, Cω9 dimethyl acetal (DMA) and C DMA. The G+C content of the genomic DNA was 68.4 mol%. On the basis of these data, strain 2CBEGH3 represents a novel species in a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is 2CBEGH3 (=JCM 31932=DSM 105194).

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2018-04-01
2024-03-19
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