1887

Abstract

A Gram-reaction-negative, strictly aerobic, milky-white and rod-shaped bacterium (designated Gsoil 115) isolated from ginseng field soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 115 grew optimally at 30 °C and at pH 7.0 on Reasoner’s 2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gsoil 115 belongs to the genus and was most closely related to B717-2 (98.6 %), 34-P (98.3 %), NBRC 106434 (98.1 %), CCUG 39402 (97.7 %) and Cr4-35 (97.5 %). The DNA G+C content was 60.9 mol%. The DNA–DNA hybridization relatedness between strain Gsoil 115 and B717-2, 34-P, NBRC 106434, . CCUG 39402 and Cr4-35 were 31.2, 21.6, 16.9, 8.7 and 10.1 %, respectively. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The sole respiratory quinone was Q-8. The major fatty acids were C and summed feature 3 (Cω7 and/or C 6), which supported the affiliation of strain Gsoil 115 to the genus . Moreover, the physiological, biochemical and low level of DNA–DNA relatedness value allowed the phenotypic and genotypic differentiation of strain Gsoil 115 from the recognized species of the genus . Therefore, strain Gsoil 115 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain Gsoil 115 (LMG 23393=KCTC 12577).

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2018-05-01
2024-03-29
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