1887

Abstract

A novel actinobacterial strain, designated C12CA1, was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1 contained -2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1 was classified in the genus . It contained MK-9(H) as the predominant menaquinone, C, iso-C and iso-C as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1 was closely related to DSM 44592 (98.96 %) and JCM 12673 (98.82 %). The strain exhibited low DNA–DNA relatedness values with DSM 44592 (6.9±0.2–11.6±1.9 %) and JCM 12673 (8.8±0.3–9.2±1.8 %). The DNA G+C content of strain C12CA1 was 69.8 mol%. Based on the results of polyphasic characterization, strain C12CA1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C12CA1 (=TBRC 1456=NBRC 111116).

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2018-05-01
2024-03-29
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