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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 5

sp. nov., isolated from rice rhizosphere soil Free

Abstract

A novel actinomycete, designated strain DH51B-4-3, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil. The isolate contained -diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain DH51B-4-3 were arabinose, galactose, glucose, rhamnose and ribose. The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The major menaquinone was MK-9(H). The main cellular fatty acids were iso-C and cyclo-C. The G+C content of the genomic DNA was 68.2 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3 should be classified in the genus and closely related to YIM 75904 (98.06 %) and DSM 44468 (97.77 %). The result of DNA–DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3 could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is DH51B-4-3 (=TBRC 6029=NBRC 112509).

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  • Accepted:
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Keyword(s): 16S rRNA gene , actinomycetes , Amycolatopsis and rice rhizosphere soil
© 2018 IUMS
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2018-05-01
2024-04-19
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Amycolatopsis rhizosphaerae sp. nov., isolated from rice rhizosphere soil
Int J Syst Evol Microbiol 68, 1546 (2018); https://doi.org/10.1099/ijsem.0.002704
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