@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002704, author = "Thawai, Chitti", title = "Amycolatopsis rhizosphaerae sp. nov., isolated from rice rhizosphere soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "5", pages = "1546-1551", doi = "https://doi.org/10.1099/ijsem.0.002704", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002704", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "rice rhizosphere soil", keywords = "16S rRNA gene", keywords = "Amycolatopsis", keywords = "actinomycetes", abstract = "A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose. The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The major menaquinone was MK-9(H4). The main cellular fatty acids were iso-C16 : 0 and cyclo-C17 : 0. The G+C content of the genomic DNA was 68.2 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06 %) and Amycolatopsis sacchari DSM 44468T (97.77 %). The result of DNA–DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp. nov. is proposed. The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).", }