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Volume 68, Issue 5

sp. nov., isolated from bamboo () litter Free

Abstract

Three actinomycetes strains, designated JBL-5, JBL-14 and JBL-20, were isolated from bamboo () litter in Damyang, Republic of Korea. Morphological, chemotaxonomic and phylogenetic analyses demonstrated that the three strains belong to the genus . Microscopic observation revealed that the three strains produced spore chains with smooth surfaces. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that these strains showed the highest sequence similarity to NRRL B-2928 (97.8 %), LMG 20322 (97.7 %), NBRC 13049 (97.7 %), NRRL B-3981 (97.7 %) and NBRC 12889 (97.7 %). The DNA–DNA hybridization values between strains JBL-5, JBL-14 and JBL-20 were 91.2–92.4 %, and the values between the three strains and their close phylogenetic relatives were also below 70 %. The predominant menaquinones were MK-9 (H) and MK-9 (H). The cell wall contained -diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositolmannoside, two unidentified aminolipids, three unidentified aminophospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C, anteiso-C, iso-C, C 7 and iso-C. The DNA G+C contents were 71.8–72.4 mol%. On the basis of phylogenetic analyses and physiological and biochemical characterization, strains JBL-5, JBL-14 and JBL-20 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBL-20 (=KACC 18269=NBRC 110629).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bamboo (Sasa borealis) litter and Streptomyces fuscigenes sp. nov.
© 2018 IUMS
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2018-05-01
2024-04-20
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Streptomyces fuscigenes sp. nov., isolated from bamboo (Sasa borealis) litter
Int J Syst Evol Microbiol 68, 1541 (2018); https://doi.org/10.1099/ijsem.0.002706
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