%0 Journal Article %A López, G. %A Díaz-Cárdenas, C. %A David Alzate, J. %A Gonzalez, L. N. %A Shapiro, N. %A Woyke, T. %A Kyrpides, N. C. %A Restrepo, S. %A Baena, S. %T Description of Alicyclobacillus montanus sp. nov., a mixotrophic bacterium isolated from acidic hot springs %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 5 %P 1608-1615 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002718 %K Firmicutes %K 16S rRNA %K Alicyclobacillaceae %K Alicyclobacillus montanus %K gyrB %I Microbiology Society, %X Three morphologically similar thermo-acidophilic strains, USBA-GBX-501, USBA-GBX-502 and USBA-GBX-503T, were isolated from acidic thermal springs at the National Natural Park Los Nevados (Colombia). All isolates were spore-forming, Gram-stain-positive and motile, growing aerobically at 25–55 °C (optimum ~45 °C) and at pH 1.5–4.5 (optimum pH ~3.0). Phylogenetic analysis of the 16S rRNA gene sequences of these isolates showed an almost identical sequence (99.0 % similarity) and they formed a robust cluster with the closest relative Alicyclobacillus tolerans DSM 16297T with a sequence similarity of 99.0 %. Average similarity to other species of the genus Alicyclobacillus was 93.0 % and average similarity to species of the genus Effusibacillus was 90 %. In addition, the level of DNA–DNA hybridization between strain USBA-GBX-503T and Alicyclobacillus tolerans DSM 16297T was 31.7 %. The genomic DNA G+C content of strain USBA-GBX-503T was 44.6 mol%. The only menaquinone was MK-7 (100.0 %). No ω-alicyclic fatty acids were detected in strain USBA-GBX-503T, and the major cellular fatty acids were C18 : 1ω7c, anteiso-C17 : 0 and iso-C17 : 0. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA–DNA relatedness values, along with low levels of identity at the whole genome level (ANIb and ANIm values of <67.0 and <91.0 %, respectively), it can be concluded that strain USBA-GBX-503T represents a novel species of the genus Alicyclobacillus , for which the name Alicyclobacillus montanus sp. nov. is proposed. The type strain is USBA-GBX-503T (=CMPUJ UGB U503T=CBMAI1927T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002718