1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 5

gen. nov., sp. nov., a new member of the family , isolated from human gut Free

Abstract

Urolithins are gut microbial metabolites that exert health benefits and are generated from ellagic acid (EA) and ellagitannin-containing foods such as strawberries, pomegranates and walnuts. species produce some intermediary urolithins but the micro-organisms responsible for the transformation of EA into the final and more bioactive urolithins, such as urolithin A and isourolithin A, are unknown. We report here a new bacterium, capable of metabolizing EA into isourolithin A, isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods. Strain CEBAS 4A belongs to the family and differed from other genera of this family, both phylogenetically and phenotypically. Based on 16S rRNA gene sequence similarity, the strain was related to DSM 19490 (92.9 % similarity), DSM 21839 (92.7 % similarity), DSM 19450 (92.5 % similarity), DSM 18785 (92.5 % similarity) and DSM 22242 (91.2 % similarity). This strain was strictly anaerobic and Gram-stain-positive. The whole-cell fatty acids were saturated (98.3 %), a very high percentage that differs from the nearest genera ranging from 62 to 73 %. The major respiratory lipoquinone was menaquinone-7 and the diamino acid in the peptidoglycan was -diaminopimelic acid. Diphosphatidylglycerol and phosphatidylglycerol comprised the main polar lipid profile in addition to several phosphoglycolipids (PGL1–2), phospholipids (PL1–4), glycolipids (GL1–6) and lipids. Based on these data, a new genus, gen. nov. is proposed with one species, sp. nov. The type strain of is CEBAS 4A (=DSM 104140=CCUG 70284).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ellagic acid , ellagitannin , gut microbiota , isourolithin A , polyphenol metabolism and urolithin
© 2018 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002735
2018-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/5/1707.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002735&mimeType=html&fmt=ahah

References

  1. Tomás-Barberán FA, González-Sarrías A, García-Villalba R, Núñez-Sánchez MA, Selma MV et al. Urolithins, the rescue of "old" metabolites to understand a "new" concept: metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status. Mol Nutr Food Res 2017; 61:1500901 [View Article][PubMed]
     [Google Scholar]
  2. Tomás-Barberán FA, Selma MV, Espín JC. Interactions of gut microbiota with dietary polyphenols and consequences to human health. Curr Opin Clin Nutr Metab Care 2016; 19:471–476 [View Article][PubMed]
     [Google Scholar]
  3. Selma MV, Espín JC, Tomás-Barberán FA. Interaction between phenolics and gut microbiota: role in human health. J Agric Food Chem 2009; 57:6485–6501 [View Article][PubMed]
     [Google Scholar]
  4. Cerdá B, Espín JC, Parra S, Martínez P, Tomás-Barberán FA. The potent in vitro antioxidant ellagitannins from pomegranate juice are metabolised into bioavailable but poor antioxidant hydroxy-6H-dibenzopyran-6-one derivatives by the colonic microflora of healthy humans. Eur J Nutr 2004; 43:205–220 [View Article][PubMed]
     [Google Scholar]
  5. Larrosa M, García-Conesa MT, Espín JC, Tomás-Barberán FA. Ellagitannins, ellagic acid and vascular health. Mol Aspects Med 2010; 31:513–539 [View Article][PubMed]
     [Google Scholar]
  6. Espín JC, Larrosa M, García-Conesa MT, Tomás-Barberán F. Biological significance of urolithins, the gut microbial ellagic acid-derived metabolites: the evidence so far. Evid Based Complement Alternat Med 2013; 2013:1–15 [View Article][PubMed]
     [Google Scholar]
  7. Tomás-Barberán FA, García-Villalba R, González-Sarrías A, Selma MV, Espín JC. Ellagic acid metabolism by human gut microbiota: consistent observation of three urolithin phenotypes in intervention trials, independent of food source, age, and health status. J Agric Food Chem 2014; 62:6535–6538 [View Article][PubMed]
     [Google Scholar]
  8. Romo-Vaquero M, García-Villalba R, González-Sarrías A, Beltrán D, Tomás-Barberán FA et al. Interindividual variability in the human metabolism of ellagic acid: contribution of Gordonibacter to urolithin production. J Funct Foods 2015; 17:785–791 [View Article]
     [Google Scholar]
  9. Selma MV, Romo-Vaquero M, García-Villalba R, González-Sarrías A, Tomás-Barberán FA et al. The human gut microbial ecology associated with overweight and obesity determines ellagic acid metabolism. Food Funct 2016; 7:1769–1774 [View Article][PubMed]
     [Google Scholar]
  10. Selma MV, Beltrán D, García-Villalba R, Espín JC, Tomás-Barberán FA. Description of urolithin production capacity from ellagic acid of two human intestinal Gordonibacter species. Food Funct 2014; 5:1779–1784 [View Article][PubMed]
     [Google Scholar]
  11. Selma MV, Tomás-Barberán FA, Beltrán D, García-Villalba R, Espín JC. Gordonibacter urolithinfaciens sp. nov., a urolithin-producing bacterium isolated from the human gut. Int J Syst Evol Microbiol 2014; 64:2346–2352 [View Article][PubMed]
     [Google Scholar]
  12. Espín JC, González-Sarrías A, Tomás-Barberán FA. The gut microbiota: a key factor in the therapeutic effects of (poly)phenols. Biochem Pharmacol 2017; 139:82–93 [View Article][PubMed]
     [Google Scholar]
  13. Selma MV, Beltrán D, Luna MC, Romo-Vaquero M, García-Villalba R et al. Isolation of human intestinal bacteria capable of producing the bioactive metabolite isourolithin A from ellagic acid. Front Microbiol 2017; 8:1–8 [View Article][PubMed]
     [Google Scholar]
  14. García-Villalba R, Espín JC, Tomás-Barberán FA. Chromatographic and spectroscopic characterization of urolithins for their determination in biological samples after the intake of foods containing ellagitannins and ellagic acid. J Chromatogr A 2016; 1428:162–175 [View Article][PubMed]
     [Google Scholar]
  15. Doetsch RN. Determinative methods of light microscopy. In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA et al. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 21–33
     [Google Scholar]
  16. Arahal DR, Sánchez E, Macián MC, Garay E. Value of recN sequences for species identification and as a phylogenetic marker within the family "Leuconostocaceae". Int Microbiol 2008; 11:33–39[PubMed]
     [Google Scholar]
  17. Lucena T, Pascual J, Garay E, Arahal DR, Macián MC et al. Haliea mediterranea sp. nov., a marine gammaproteobacterium. Int J Syst Evol Microbiol 2010; 60:1844–1848 [View Article][PubMed]
     [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  19. Jukes TH, Cantor CR. Evolution of Protein Molecules: Mammalian Protein Metabolism New York: Academic Press; 1969 pp. 21–132 [Crossref]
     [Google Scholar]
  20. Rhuland LE, Work E, Denman RF, Hoare DS. The behavior of the isomers of α,ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc 1955; 77:4844–4846 [View Article]
     [Google Scholar]
  21. Würdemann D, Tindall BJ, Pukall R, Lünsdorf H, Strömpl C et al. Gordonibacter pamelaeae gen. nov., sp. nov., a new member of the Coriobacteriaceae isolated from a patient with Crohn's disease, and reclassification of Eggerthella hongkongensis Lau et al. 2006 as Paraeggerthella hongkongensis gen. nov., comb. nov. Int J Syst Evol Microbiol 2009; 59:1405–1415 [View Article][PubMed]
     [Google Scholar]
  22. Maruo T, Sakamoto M, Ito C, Toda T, Benno Y. Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella . Int J Syst Evol Microbiol 2008; 58:1221–1227 [View Article][PubMed]
     [Google Scholar]
  23. Clavel T, Charrier C, Wenning M, Haller D. Parvibacter caecicola gen. nov., sp. nov., a bacterium of the family Coriobacteriaceae isolated from the caecum of a mouse. Int J Syst Evol Microbiol 2013; 63:2642–2648 [View Article][PubMed]
     [Google Scholar]
  24. Lagier JC, Elkarkouri K, Rivet R, Couderc C, Raoult D et al. Non contiguous-finished genome sequence and description of Senegalemassilia anaerobia gen. nov., sp. nov. Stand Genomic Sci 2013; 7:343–356 [View Article][PubMed]
     [Google Scholar]
  25. Gupta RS, Chen WJ, Adeolu M, Chai Y. Molecular signatures for the class Coriobacteriia and its different clades; proposal for division of the class Coriobacteriia into the emended order Coriobacteriales, containing the emended family Coriobacteriaceae and Atopobiaceae fam. nov., and Eggerthellales ord. nov., containing the family Eggerthellaceae fam. nov. Int J Syst Evol Microbiol 2013; 63:3379–3397 [View Article][PubMed]
     [Google Scholar]
  26. Yarza P, Richter M, Peplies J, Euzeby J, Amann R et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 2008; 31:241–250 [View Article][PubMed]
     [Google Scholar]
  27. Clavel T, Lepage P, Charrier C. The family Coriobacteriaceae . In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F et al. (editors) The Prokaryotes-Actinobacteria Berlin: Springer-Verlag; 2014 pp. 201–238
     [Google Scholar]
  28. Clavel T, Charrier C, Braune A, Wenning M, Blaut M et al. Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov. Int J Syst Evol Microbiol 2009; 59:1805–1812 [View Article][PubMed]
     [Google Scholar]
  29. Clavel T, Duck W, Charrier C, Wenning M, Elson C et al. Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009. Int J Syst Evol Microbiol 2010; 60:1527–1531 [View Article][PubMed]
     [Google Scholar]
  30. Minamida K, Ota K, Nishimukai M, Tanaka M, Abe A et al. Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum. Int J Syst Evol Microbiol 2008; 58:1238–1240 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002735
Loading
Ellagibacter isourolithinifaciens gen. nov., sp. nov., a new member of the family Eggerthellaceae, isolated from human gut
Int J Syst Evol Microbiol 68, 1707 (2018); https://doi.org/10.1099/ijsem.0.002735
/content/journal/ijsem/10.1099/ijsem.0.002735
/content/journal/ijsem/10.1099/ijsem.0.002735
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error