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Campylobacter blaseri sp. nov., isolated from common seals (Phoca vitulina)

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  • Authors: Maarten J. Gilbert1​,2​ ,  Aldert L. Zomer1​,3​ ,  Arjen J. Timmerman1​ ,  Mirlin P. Spaninks1​ ,  Ana Rubio-García1​,4​,5​ ,  John W. Rossen4​ ,  Birgitta Duim1​,3​ , Jaap A. Wagenaar1​,3​,6​
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    • 1​ 1​Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands 2​ 2​Reptile, Amphibian and Fish Conservation Netherlands (RAVON), Nijmegen, The Netherlands 3​ 3​WHO Collaborating Centre for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands 4​ 4​Sealcentre, Pieterburen, The Netherlands 5​ 5​Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands 6​ 6​Wageningen Bioveterinary Research, Lelystad, The Netherlands
  • *Correspondence: Maarten J. Gilbert [email protected]
  • First Published Online: 06 April 2018, International Journal of Systematic and Evolutionary Microbiology 68: 1787-1794, doi: 10.1099/ijsem.0.002742
  • Subject: New Taxa - Proteobacteria
  • Received:
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  • During a study to assess the faecal microbiome of common seals (Phoca vitulina) in a Dutch seal rehabilitation centre, 16S rRNA gene sequences of an unknown Campylobacter taxon were identified. Campylobacter isolates, which differed from the established Campylobacter taxa, were cultured and their taxonomic position was determined by a polyphasic study based on ten isolates. The isolates were characterized by 16S rRNA and atpA gene sequence analyses and by conventional phenotypic testing. Based on the whole genome sequences, the average nucleotide identity and core genome phylogeny were determined. The isolates formed a separate phylogenetic clade, divergent from all other Campylobacter taxa and most closely related to Campylobacter corcagiensis , Campylobacter geochelonis and Campylobacter ureolyticus . The isolates can be distinguished phenotypically from all other Campylobacter taxa based on their lack of motility, growth at 25 °C and growth on MacConkey agar. This study shows that these isolates represent a novel species within the genus Campylobacter , for which the name Campylobacter blaseri sp. nov. is proposed. The type strain for this novel species is 17S00004-5 (=LMG 30333=CCUG 71276).

  • The GenBank accession numbers for the whole genome sequences of isolates 17S00004-5 and 17S00008-12 are PDHH00000000 and PDHI00000000, respectively. The GenBank accession numbers for the atpA sequences of isolates 17S00004-5 and 17S00008-12 are MG958595 and MG958596, respectively. The GenBank accession numbers for the 16S rRNA sequences of isolates 17S00004-5, 17S00008-12, 17S01633-4, 17S01636-3, 17S01637-3, 17S01639-3, 17S01643-3, 17S01644-3, 17S01646-3, and 17S01649-5 are MG013475, MG013476, MG013477, MG013478, MG013479, MG013480, MG013481, MG013483, MG013485 and MG013486, respectively.

  • One supplementary figure is available with the online version of this article.

© 2018 IUMS | Published by the Microbiology Society

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/content/journal/ijsem/10.1099/ijsem.0.002742
2018-04-06
2019-02-15
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